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Figure 4: (a) Representative Immunoblot images demonstrating the effect of SYA on NQO1, Nrf2, Atg7, and p62 in NRK 52E cells challenged with high glucose. (b) Immunofluorescence images of NRK 52E cells immunostained with LC-3 and Lysotracker red. (c) Immunofluorescence images depicting immunostaining of Nrf2 in NRK 52E cells. The values are expressed in terms of mean ± SEM (n = 3). NC = Normal NRK 52E cells, NC + SYA-20: Normal NRK 52E cells treated with SYA at 20 μM HG-NRK 52E cells challenged with 30 mM glucose. SYA = Syringic acid, LC: Light chain, SEM = Standard error of the mean, NRK: Normal rat kidney, Nrf2 = Nuclear factor erythroid 2-related factor 2

Figure 4: (a) Representative Immunoblot images demonstrating the effect of SYA on NQO1, Nrf2, Atg7, and p62 in NRK 52E cells challenged with high glucose. (b) Immunofluorescence images of NRK 52E cells immunostained with LC-3 and Lysotracker red. (c) Immunofluorescence images depicting immunostaining of Nrf2 in NRK 52E cells. The values are expressed in terms of mean ± SEM (<i>n</i> = 3). NC = Normal NRK 52E cells, NC + SYA-20: Normal NRK 52E cells treated with SYA at 20 μM HG-NRK 52E cells challenged with 30 mM glucose. SYA = Syringic acid, LC: Light chain, SEM = Standard error of the mean, NRK: Normal rat kidney, Nrf2 = Nuclear factor erythroid 2-related factor 2