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Figure 4: Control groups (a and b) normal control, (g and h gossypin control) show no or minimal expression of caspase 3and NFκ-B respectively. Anticaspase 3 and anti-NFκ-B immune-stained sections in the liver, ×400. Toxic group (c and i) showing intense brown nuclear immunostaining of caspase 3 and NFκ-B (arrows). Treated group (d and j) shows a marked decrease in the expression × 400. (e and f) caspase 3 and NFκ-B immunostaining scores, respectively. *,#Significantly different from control, MTX, and MTX-gossypin-treated groups, respectively, at P < 0.05. MTX = methotrexate, NFκ-B = Nuclear factor kappa-B

Figure 4:  Control groups (a and b) normal control, (g and h gossypin control) show no or minimal expression of caspase 3and NFκ-B respectively. Anticaspase 3 and anti-NFκ-B immune-stained sections in the liver, ×400. Toxic group (c and i) showing intense brown nuclear immunostaining of caspase 3 and NFκ-B (arrows). Treated group (d and j) shows a marked decrease in the expression × 400. (e and f) caspase 3 and NFκ-B immunostaining scores, respectively. *<sup>,#</sup>Significantly different from control, MTX, and MTX-gossypin-treated groups, respectively, at <i>P</i> < 0.05. MTX = methotrexate, NFκ-B = Nuclear factor kappa-B