Figure 2: Fluorescence polarization-based ligand screen assay was done to analyze the affinity of genistein for peroxisome proliferator-activated receptor gamma and estrogen receptor beta and EC50 values for the genistein and the respective positive controls were measure by plotting a graph between concentration (pM) and milli-polarization unit (mP), (a) demonstrating the genistein and pioglitazone affinity against peroxisome proliferator-activated receptor gamma whereas, (b) represents affinity of genistein and estradiol against estrogen receptor beta, (c) shows the blood glucose levels of diabetic rats at different time interval in the whole study, (d) represents the percentage elevation in blood glucose level in diabetic untreated, pioglitazone 3.5 mg/kg treated, Genistein 30 mg/kg and 60 mg/kg treated groups (treatment vs. diabetic control P < 0.001, ANOVA) (e) was obtained from pharmacokinetic analysis of genistein, plasma concentration versus time curve observed (•) and software predicted (−), plasma concentration over the 7 days of experimental time points was plotted