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Figure 1: (a) The effects of the trypsin inhibitors on the proliferation of human embryonic kidney and A549 cells. Cells were maintained in RPMI 1640 medium containing 10% fetal bovine serum, and antibiotics, at 37°C, 5% CO2 humid condition. Confluent cells (5 × 103 cells/well) were incubated with different trypsin inhibitor at 10 μg/ml concentration for 24 h and 48 h, and % cytotoxicity was determined using the MTT assay, (b) the effects of the trypsin inhibitors on the proliferation of human embryonic kidney and A549 cells. Cells were maintained in RPMI 1640 medium containing 10% fetal bovine serum, and antibiotics, at 37°C, 5% CO2 humid condition. Confluent cells (5 × 103 cells/well) were incubated with different trypsin inhibitor at final concentration of 5 μg/ml for 24 h and 48 h, and % cytotoxicity was determined using the MTT assay

Figure 1: (a) The effects of the trypsin inhibitors on the proliferation of human embryonic kidney and A549 cells. Cells were maintained in RPMI 1640 medium containing 10% fetal bovine serum, and antibiotics, at 37°C, 5% CO<sub>2</sub> humid condition. Confluent cells (5 × 10<sup>3</sup> cells/well) were incubated with different trypsin inhibitor at 10 μg/ml concentration for 24 h and 48 h, and % cytotoxicity was determined using the MTT assay, (b) the effects of the trypsin inhibitors on the proliferation of human embryonic kidney and A549 cells. Cells were maintained in RPMI 1640 medium containing 10% fetal bovine serum, and antibiotics, at 37°C, 5% CO<sup>2</sup> humid condition. Confluent cells (5 × 10<sup>3</sup> cells/well) were incubated with different trypsin inhibitor at final concentration of 5 μg/ml for 24 h and 48 h, and % cytotoxicity was determined using the MTT assay