Figure 4: Photomicrographsof nNOS, iNOS and eNOS staining within the control and IBS tissues is depicted (×10). The immunoreactivity of sections stained with (a and b) nNOS (c and d) iNOS and (e and f) eNOS primary antibodies. Intensity of reactivity (score: 0= no staining; 1= mild; 2= moderate, and; 3= intense). nNOS: Neuronal nitric oxide synthase, iNOS: Inducible nitric oxide synthase, eNOS: Endothelial nitric oxide synthase