Figure 5: Immunohistochemical analysis of PepT1 expression on intestinal membrane (a) Vehicle control group – duodenum; (b) Vehicle control group – jejunum (c) methotrexate-treated group – duodenum; (d) methotrexate-treated group – jejunum Key: Ag/Ab = Antigen-Antibody reaction showing presence of PepT1 in apical membrane of villi, V = Villi, C = Crypts and VC = Villous Collapse