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Figure 2: Vascular endothelial growth factor (VEGF) and pigment epithelial derived factor (PEDF) gene expression after exposing with various concentration of rAPN. (a) Real time (RT) polymerase chain reaction for VEGF, PEDF in retinal pigment epithelial (RPE) cells after exposing with various concentration of rAPN. Lane 1: 100– 600 bp ladders, Lane 2: Control, Lane 3– 6: RPE exposed to 5 pg, 50 pg, 500 pg, 5000 pg rAPN for 1 h. (b) RT polymerase chain reaction for the mRNA expression of VEGF, PEDF in human retinal pigment epithelial cell lines cells exposed to varying concentration of rAPN. rAPN exposed cells showed a significant decrease in VEGF, signifi cant increase in PEDF (*statistically significant compared to control)

Figure 2: Vascular endothelial growth factor (VEGF) and pigment epithelial derived factor (PEDF) gene expression after exposing with various concentration of rAPN. (a) Real time (RT) polymerase chain reaction for VEGF, PEDF in retinal pigment epithelial (RPE) cells after exposing with various concentration of rAPN. Lane 1: 100– 600 bp ladders, Lane 2: Control, Lane 3– 6: RPE exposed to 5 pg, 50 pg, 500 pg, 5000 pg rAPN for 1 h. (b) RT polymerase chain reaction for the mRNA expression of VEGF, PEDF in human retinal pigment epithelial cell lines cells exposed to varying concentration of rAPN. rAPN exposed cells showed a significant decrease in VEGF, signifi cant increase in PEDF (*statistically significant compared to control)