Figure 4: The translocation of p-Smad2/3 in cardiac fibroblasts with different treatments determined using immunocytochemistry. 1, Control; 2, 5 ng/mL (transforming growth factor beta 1 (TGFβ1) stimulation for 15 min; 3, 5 ng/mL TGFβ1 stimulation for 60 min; 4, 5 ng/mL TGFβ1 stimulation for 120 min; 5, 10−6 mol/L tanshinone (TSN) pretreatment + 5 ng/mL TGFβ1 stimulation; 6, 10−5 mol/L TSN pretreatment + 5 ng/mL TGFβ1 stimulation; 7, 10−4 mol/L TSN pretreatment + 5 ng/mL TGFβ1 stimulation
