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Figure 2: Induction of interferon (IFN)-g and tumor necrosis factor (TNF)-aproduction in murine NK cells by KLH in vitro. Murine NK cells enriched by negative selection were incubated for 24 h with or without 25 mg/ml of KLH or 5 U/ml of interleukin (IL)-2. The levels of IFN-g and TNF-ain cultured supernatants were measured by an enzyme-linked immunosorbent assay (ELISA). The data are means ± SEM of three independent experiments. **P < 0.01 and ***P < 0.001, as compared with the values of control cultures incubated in the medium alone (Dunnett's t-test) |
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