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Figure 7: The effects of varying the injection volume (a) 40 μl (b) 20 μl. The mobile phase used was 33:67 MeOH:Heptanesulfonic sodium salt (0.05mM) with 0.05% TEA, pH 2.3, running at 0.5 ml/min. The detection was done at 210 nm

Figure 7: The effects of varying the injection volume (a) 40 μl (b) 20 μl. The mobile phase used was 33:67 MeOH:Heptanesulfonic sodium salt (0.05mM) with 0.05% TEA, pH 2.3, running at 0.5 ml/min. The detection was done at 210 nm