Figure 5: (i) The effect of using ACN as organic solvent in mobile phase. (ii) The effects of varying the percentage of organic solvent: (a) 37% MeOH (b) 33% MeOH (c) 30% MeOH (d) 25% MeOH (e) 40% MeOH (f) 35% MeOH. The mobile phase used was 0.05 mM heptanesulfonic sodium salt with 0.05% TEA, pH 2.3, running at 0.3 ml/min. The detection was done at 210 nm