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Figure 4: The effects of adding different concentrations of organic modifier:TEA (a) 0.05% TEA, (b) 0.07% TEA, (c) 0.1% TEA, (d) 0.75% TEA, (e) 1% TEA, and (f) 0.5%. The mobile used was 25:75 MeOH:Heptanesulfonic sodium salt (0.05 mM) pH 2.8, running at 0.5 ml/min. The detection was done at 210 nm |
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