Figure 3: Effect of HAE (10– 100 mg/kg), EAE (10– 100 mg/kg), PEE (10– 100 mg/kg) and morphine (0.3-3 mg/kg) on the time course curves (a, c, e) and the total antinociceptive effect (calc. as AUCs) (b, c, f) in the hot plate test in mice. Data are presented as mean ± S.E.M. (n = 5). The lower and upper margins of the boxes (b, d, f) represent the 25 th and 75 th percentiles, with the extended arms representing the 10 th and 90 th percentiles, respectively. The median is shown as the horizontal line within the box..*P < 0.05, **P < 0.01, ***P < 0.001 compared to the control group (ctrl) (two-way repeated measures ANOVA followed by Bonferroni's post hoc). †P < 0.05, ††P < 0.01, †††P < 0.001 compared to the control group (ctrl) (one-way ANOVA followed by Newman– Keuls post hoc).