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Figure 2: Effect of BP-H on NO production and iNOS protein in LPS-induced RAW 264.7 cells. RAW cells were treated with 10, 30, 50 and 80 μg/ml of BP-H and LPS (100 ng/ml) for 24 hr. After incubation, the culture medium was collected for nitrite assay and cell lysates were subjected to SDS-PAGE for iNOS measurement. Changes in iNOS protein levels normalized by β-actin were quantified. The data are the mean ± S.E.M. from at least three independent experiments, each performed in duplicate. The asterisks denote the significance levels when compared to control groups (* P<0.05)

Figure 2: Effect of BP-H on NO production and iNOS protein in LPS-induced RAW 264.7 cells. RAW cells were treated with 10, 30, 50 and 80 μg/ml of BP-H and LPS (100 ng/ml) for 24 hr. After incubation, the culture medium was collected for nitrite assay and cell lysates were subjected to SDS-PAGE for iNOS measurement. Changes in iNOS protein levels normalized by β-actin were quantified. The data are the mean ± S.E.M. from at least three independent experiments, each performed in duplicate. The asterisks denote the significance levels when compared to control groups (* <i>P</i><0.05)