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Figure 2: 15% SDS-polyacrylamide gel electrophoresis of the proteins released from the chromatin (a) and nuclei (b) upon titration with mitoxantrone. (b), Lanes 1--4 are 0, 10, 20, and 40 μM of mitoxantrone, respectively. (b) Lanes 3-8 are 0, 10, 20, 40, 80, 160, and 220 μM of drug, respectively. Lanes 1and 2 are liver histone and HMG proteins as a standard proteins (n=3). The gels were blotted with histones antibody (c) and H1º antibody (d). Lane 1 is liver histone proteins and lanes 2-5 are nuclei treated with 0, 40, 80, and 160 μM of mitoxantrone. Number of experiments was 3

Figure 2: 15% SDS-polyacrylamide gel electrophoresis of the proteins released from the chromatin (a) and nuclei (b) upon titration with mitoxantrone. (b), Lanes 1--4 are 0, 10, 20, and 40 μM of mitoxantrone, respectively. (b) Lanes 3-8 are 0, 10, 20, 40, 80, 160, and 220 μM of drug, respectively. Lanes 1and 2 are liver histone and HMG proteins as a standard proteins (n=3). The gels were blotted with histones antibody (c) and H1º antibody (d). Lane 1 is liver histone proteins and lanes 2-5 are nuclei treated with 0, 40, 80, and 160 μM of mitoxantrone. Number of experiments was 3