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Year : 2022  |  Volume : 54  |  Issue : 3  |  Page : 171--176

High-level gentamicin resistance mediated by Aac(6′)-Ie-aph(2”)-Ia gene in Enterococcus species isolated from clinical samples in Northern India

Ayan Kumar Das1, Mridu Dudeja1, Sunil Kohli2, Pratima Ray3, Shyamasree Nandy1 
1 Department of Microbiology, Hamdard Institute of Medical Sciences and Research, Jamia Hamdard University, New Delhi, India
2 Medicine, Hamdard Institute of Medical Sciences and Research, Jamia Hamdard University, New Delhi, India
3 Department of Biotechnology, School of Chemical and Life Sciences, Jamia Hamdard University, New Delhi, India

Correspondence Address:
Dr. Ayan Kumar Das
Department of Microbiology, Hamdard Institute of Medical Sciences and Research, Jamia Hamdard University, New Delhi - 110 062
India

CONTEXT: Enterococci are known to cause life-threatening infections which are difficult to treat as the organism harbors innate resistance to many antibiotics and can amass resistance toward many others through plasmid-mediated genetic exchange. AIMS: The study evaluates the drug susceptibility profile of various Enterococcus species isolated from various patient specimens submitted for bacteriological analysis and check the incidence of aac(6′) Ie-aph(2”) Ia gene. SETTING AND DESIGN: This in vitro cross-sectional study was executed at bacteriology laboratory of a 470 bedded hospital in New Delhi. MATERIALS AND METHODS: Drug susceptibility testing was carried out on enterococcal isolates. High-level gentamicin-resistant (HLGR) isolates detected by micro broth dilution assay were then subjected to molecular detection of aac(6′) Ie-aph(2”) Ia gene. STATISTICAL ANALYSIS USED: The level of significance was established by Chi-square test. RESULTS: Among the 182 enterococcal stains detected, 76.9% were Enterococcus faecalis and 20.3% were Enterococcus faecium. 12.08% strains were vancomycin resistant. 39% expressed resistance toward high-level gentamicin (HLG) and this finding was significantly higher in E. faecium than E. faecalis. HLGR strains expressed a higher degree of resistance to other drugs in contrast to non-HLGR isolates. In 67 out of 71 HLGR isolates the bifunctional gene was detected. CONCLUSION: Considerable presence of HLG and vancomycin resistance in the clinical isolates is alarming and should be taken seriously. The study shows high dissemination of aac(6′)-Ie-aph(2”)-Ia gene among Enterococci isolated from the region.


How to cite this article:
Das AK, Dudeja M, Kohli S, Ray P, Nandy S. High-level gentamicin resistance mediated by Aac(6′)-Ie-aph(2”)-Ia gene in Enterococcus species isolated from clinical samples in Northern India.Indian J Pharmacol 2022;54:171-176


How to cite this URL:
Das AK, Dudeja M, Kohli S, Ray P, Nandy S. High-level gentamicin resistance mediated by Aac(6′)-Ie-aph(2”)-Ia gene in Enterococcus species isolated from clinical samples in Northern India. Indian J Pharmacol [serial online] 2022 [cited 2022 Aug 12 ];54:171-176
Available from: https://www.ijp-online.com/article.asp?issn=0253-7613;year=2022;volume=54;issue=3;spage=171;epage=176;aulast=Das;type=0