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Year : 2021  |  Volume : 53  |  Issue : 1  |  Page : 13--18

Determination of in vitro human whole blood-to-plasma ratio of THJ-018 utilizing gas chromatography–Mass spectrometry

Sachil Kumar1, Remal Nahar Khleel Alkhibery1, Abdulsallam Bakdash1, Mohamed Sultan Mohamed Abdelhady2 
1 Department of Forensic Sciences, College of Criminal Justice, Naif Arab University for Security Sciences, Riyadh, Saudi Arabia
2 Riyadh Poisons Control & Medical Forensic Chemistry Center, Kaab Ibn Al Harith, Ar Rawdah, Riyadh, Saudi Arabia; Egyptian Drug Authority, National Organizations for Drug Control and Research (NODCAR), Giza, Egypt

Correspondence Address:
Dr. Sachil Kumar
Assistant Professor, Department of Forensic Sciences, College of Criminal Justice, Naif Arab University for Security Sciences, Riyadh
Saudi Arabia

OBJECTIVES: This study was aimed to determine in vitro human whole blood-to-plasma ratio (KWB/P) of THJ-018 by gas chromatography/mass spectrometry (GC/MS). MATERIALS AND METHODS: The samples (human blood) were sprayed with THJ-018 and an internal standard and extracted using solid-phase extraction. THJ-018 was determined in the final extracts by GC/MS. RESULTS: The value for KWB/P was 1.56 (1.38–1.81), and red blood cell partitioning was 1.01 (1.01–1.02). The distribution of THJ-018 between whole blood and plasma was observed to be affected by temperature. CONCLUSION: The data analysis supports the proposition that the ratio of the plasma to whole blood concentrations (1.56) is a suitable parameter characterizing THJ-018 distribution in whole blood. For toxicological analysis, it would be best to refrain from converting any drug concentration measured in whole blood to that anticipated in plasma or serum; however, toxic and therapeutic concentrations should be determined for the individual specimens collected.


How to cite this article:
Kumar S, Khleel Alkhibery RN, Bakdash A, Mohamed Abdelhady MS. Determination of in vitro human whole blood-to-plasma ratio of THJ-018 utilizing gas chromatography–Mass spectrometry.Indian J Pharmacol 2021;53:13-18


How to cite this URL:
Kumar S, Khleel Alkhibery RN, Bakdash A, Mohamed Abdelhady MS. Determination of in vitro human whole blood-to-plasma ratio of THJ-018 utilizing gas chromatography–Mass spectrometry. Indian J Pharmacol [serial online] 2021 [cited 2022 Dec 8 ];53:13-18
Available from: https://www.ijp-online.com/article.asp?issn=0253-7613;year=2021;volume=53;issue=1;spage=13;epage=18;aulast=Kumar;type=0