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Year : 2017  |  Volume : 49  |  Issue : 2  |  Page : 161--167

Effect of karanjin on 2,4,6-trinitrobenzenesulfonic acid-induced colitis in Balb/c mice

Praful Prakash Patel1, Naitikumar Devshankar Trivedi2 
1 Department of Pharmaceutical Sciences, JJT University, Jhunjunu, Rajasthan, India
2 Department of Pharmacology, A R College of Pharmacy, Anand, Gujarat, India

Correspondence Address:
Praful Prakash Patel
Department of Pharmaceutical Sciences, JJT University, Jhunjunu, Rajasthan
India

OBJECTIVES: The objective of this study is to evaluate the beneficial effect of karanjin for the treatment of experimental colitis. METHODS: Colitis was induced in the Balb/c mice by rectal administration of 2% solution of 2,4,6-trinitrobenzenesulfonic acid (TNBS) in 50% methanol. Karanjin (>98% pure) was administered in two different concentrations 100 and 200 mg/kg and sulfasalazine (100 mg/kg) as reference for 7 consecutive days to colitic mice. On the 8 day, mice were euthanized and degree of inflammation was assessed by macroscopic, microscopic, histology and biochemical estimation of myeloperoxidase (MPO), nitric oxide (NO), malondialdehyde (MDA), catalase (CAT), superoxide dismutase (SOD), and reduced glutathione (GSH) level were measured. RESULTS: Karanjin significantly and dose dependently ameliorate the macroscopic damage, histological changes such as cellular infiltration, tissue necrosis, mucosal and submucosal damage as compared to the TNBS control group. Karanjin reduces the activity of MPO, depressed MDA, and NO level and helps in restoring the level of CAT, SOD, and GSH to normal when compared to the TNBS colitis group. CONCLUSION: Result of the present study indicates that karanjin has the potential to cure colitis induced by intracolonic administration of TNBS.


How to cite this article:
Patel PP, Trivedi ND. Effect of karanjin on 2,4,6-trinitrobenzenesulfonic acid-induced colitis in Balb/c mice.Indian J Pharmacol 2017;49:161-167


How to cite this URL:
Patel PP, Trivedi ND. Effect of karanjin on 2,4,6-trinitrobenzenesulfonic acid-induced colitis in Balb/c mice. Indian J Pharmacol [serial online] 2017 [cited 2021 Nov 27 ];49:161-167
Available from: https://www.ijp-online.com/article.asp?issn=0253-7613;year=2017;volume=49;issue=2;spage=161;epage=167;aulast=Patel;type=0