Indian Journal of Pharmacology Home 

RESEARCH ARTICLE
[View FULLTEXT] [Download PDF]
Year : 2016  |  Volume : 48  |  Issue : 1  |  Page : 64--68

Cytotoxic potential of few Indian fruit peels through 3-(4,5-dimethylthiazol-yl)-2,5-diphenyltetrazolium bromide assay on HepG2 cells

Munish Garg, Kusum Lata, Saurabh Satija 
 Department of Pharmaceutical Sciences, Maharshi Dayanand University, Rohtak, Haryana, India

Correspondence Address:
Munish Garg
Department of Pharmaceutical Sciences, Maharshi Dayanand University, Rohtak, Haryana
India

Objective: To investigate in vitro anticancer activity of a few Indian fruit peels through 3-(4,5-dimethylthiazol-yl)-2,5-diphenyltetrazolium bromide (MTT) assay against HepG2 cells. Materials and Methods: Hydroalcoholic extracts were prepared of five fruit peels, i.e., banana, lemon, guava, orange, and papaya by maceration and thereafter subjected for MTT assay to evaluate anticancer potential on HepG2 cells. Plant extract showed best activity was further fractionated with petroleum ether, chloroform, and ethyl acetate successively and screened again. Phytochemical analysis was then carried out to find out responsible components for the observed activity. Results: Out of the 40 samples from five fruit peel extracts with rich folklore usage, papaya extract showed maximum activity with least inhibitory concentration 50 (IC 50 ) value of 18.5 μg/ml. Further analysis after fractionation of the papaya peel extract, aqueous fraction showed the maximum inhibitory activity with least IC 50 value of 17.3 μg/ml. Phytochemical analysis of the aqueous fraction of papaya peel extract revealed the presence of flavonoids and glycosides. Total flavonoid content found to be 72.25 mg/g. Conclusion: Papaya fruit extract demonstrated the best activity against MTT assay which may be due to the presence of flavonoids.


How to cite this article:
Garg M, Lata K, Satija S. Cytotoxic potential of few Indian fruit peels through 3-(4,5-dimethylthiazol-yl)-2,5-diphenyltetrazolium bromide assay on HepG2 cells.Indian J Pharmacol 2016;48:64-68


How to cite this URL:
Garg M, Lata K, Satija S. Cytotoxic potential of few Indian fruit peels through 3-(4,5-dimethylthiazol-yl)-2,5-diphenyltetrazolium bromide assay on HepG2 cells. Indian J Pharmacol [serial online] 2016 [cited 2022 Jan 20 ];48:64-68
Available from: https://www.ijp-online.com/article.asp?issn=0253-7613;year=2016;volume=48;issue=1;spage=64;epage=68;aulast=Garg;type=0