Interaction potential of Trigonella foenum graceum through cytochrome P450 mediated inhibition
Sk Milan Ahmmed1, Pulok K Mukherjee1, Shiv Bahadur1, Amit Kar1, Kakali Mukherjee1, Sanmoy Karmakar1, Arun Bandyopadhyay2
1 Department of Pharmaceutical Technology, School of Natural Product Studies, Jadavpur University, Kolkata, West Bengal, India
2 Indian Institute of Chemical Biology, Jadavpur, Kolkata, India
Objective: The seeds of Trigonella foenum-graecum (TFG) (family: Leguminosae) are widely consumed both as a spice in food and Traditional Medicine in India. The present study was undertaken to evaluate the inhibitory effect of standardized extract of TFG and its major constituent trigonelline (TG) on rat liver microsome (RLM) and cytochrome P450 (CYP450) drug metabolizing isozymes (CYP3A4 and CYP2D6), which may indicate the possibility of a probable unwanted interaction.
Materials and Methods: Reverse phase-high performance liquid chromatography method was developed to standardize the hydroalcoholic seed extract with standard TG. The inhibitory potential of the extract and TG was evaluated on RLM and CYP isozymes using CYP450-carbon monoxide (CYP450-CO) complex assay and fluorescence assay, respectively.
Results: The content of TG in TFG was found to be 3.38% (w/w). The CYP-CO complex assay showed 23.32% inhibition on RLM. Fluorescence study revealed that the extract and the biomarker had some inhibition on CYP450 isozymes e.g. CYP3A4 and CYP2D6 (IC50values of the extract: 102.65 ± 2.63–142.23 ± 2.61 µg/ml and TG: 168.73 ± 4.03–180.90 ± 2.49 µg/ml) which was very less compared to positive controls ketoconazole and quinidine. Inhibition potential of TFG was little higher than TG but very less compared to positive controls.
Conclusions: From the present study, we may conclude that the TFG or TG has very less potential to inhibit the CYP isozymes (CYP3A4, CYP2D6), so administration of this plant extract or its biomarker TG may be safe.
Dr. Pulok K Mukherjee
Department of Pharmaceutical Technology, School of Natural Product Studies, Jadavpur University, Kolkata, West Bengal
|How to cite this article:|
Ahmmed SM, Mukherjee PK, Bahadur S, Kar A, Mukherjee K, Karmakar S, Bandyopadhyay A. Interaction potential of Trigonella foenum graceum through cytochrome P450 mediated inhibition.Indian J Pharmacol 2015;47:530-534
|How to cite this URL:|
Ahmmed SM, Mukherjee PK, Bahadur S, Kar A, Mukherjee K, Karmakar S, Bandyopadhyay A. Interaction potential of Trigonella foenum graceum through cytochrome P450 mediated inhibition. Indian J Pharmacol [serial online] 2015 [cited 2023 Mar 27 ];47:530-534
Available from: https://www.ijp-online.com/article.asp?issn=0253-7613;year=2015;volume=47;issue=5;spage=530;epage=534;aulast=Ahmmed;type=0