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Effect of adiponectin on expression of vascular endothelial growth factor and pigment epithelium-derived factor: An in vitro study
Vidhya Srinivasan1, KN Sulochana2
1 Research Scholar, BITS, Pilani, Rajasthan, India
2 Department of Biochemistry and Cell biology, Vision Research Foundation, Nungambakkam, Chennai, Tamil Nadu, India
Correspondence Address:
Dr. K N Sulochana
Department of Biochemistry and Cell biology, Vision Research Foundation, Nungambakkam, Chennai, Tamil Nadu
India
Aim: This study was carried out to identify the role of adiponectin (APN) in modulating the expression of vascular endothelial growth factor (VEGF) and pigment epithelial-derived factor (PEDF) in relation to ocular angiogenesis.
Materials and Methods: Human retinal pigment epithelial cell lines (ARPE-19) were cultured in Dulbeco«SQ»s minimum essential medium with 10% fetal bovine serum (FBS) and exposed to varying concentrations of recombinant adiponectin (5-5 ng/ml) for 1 h. Analysis of VEGF and PEDF mRNA was done by reverse transcriptase and further quantified by quantitative polymerase chain reaction. VEGF and PEDF protein expression were studied using enzyme-linked immune sorbent assay (ELISA).
Statistical Analysis: Unpaired Student«SQ»s t-test was used to analyze the significance. P < 0.05 was accepted as statistically significant.
Results: ARPE cells exposed to APN showed decreased expression of VEGF mRNA, protein whereas PEDF protein is unaltered and PEDF mRNA was increased.
Conclusion: Our in vitro study on ARPE exposed to APN showed a negative correlation with VEGF levels. Thus indicating the protective role for APN in angiogenesis-related diseases.
How to cite this article:
Srinivasan V, Sulochana K N. Effect of adiponectin on expression of vascular endothelial growth factor and pigment epithelium-derived factor: An in vitro study.Indian J Pharmacol 2015;47:117-120
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How to cite this URL:
Srinivasan V, Sulochana K N. Effect of adiponectin on expression of vascular endothelial growth factor and pigment epithelium-derived factor: An in vitro study. Indian J Pharmacol [serial online] 2015 [cited 2023 Jun 3 ];47:117-120
Available from: https://www.ijp-online.com/article.asp?issn=0253-7613;year=2015;volume=47;issue=1;spage=117;epage=120;aulast=Srinivasan;type=0 |
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