Evaluation of cytochrome P450 2D6 phenotyping in healthy adult Western Indians
Nithya J Gogtay1, Nitin B Mali1, Krishna Iyer2, Prashant P Kadam1, Kannan Sridharan1, Divya Shrimal1, Urmila M Thatte1
1 Department of Clinical Pharmacology, Seth G S Medical College and KEM Hospital, Mumbai, Maharashtra, India
2 Department of Pharmaceutical Chemistry, Bombay College of Pharmacy, Mumbai, Maharashtra, India
Background: Cytochrome P450 2D6 (CYP2D6) metabolizes around 25% of the drugs used in therapeutics and different polymorphisms have been identified in various populations. This study aimed at finding the prevalence of CYP2D6 polymorphisms using dextromethorphan as a probe drug.
Materials and Methods: Healthy participants were administered 60 mg dextromethorphan after an overnight fast and 5 ml of blood was collected 3 h postdose. A validated laboratory method was used to measure both dextromethorphan and its active metabolite, dextrorphan from plasma. Metabolic ratio (MR) of dextromethorphan to dextrorphan was calculated for each of the participants. Probit analysis was done and antimode was defined. Individuals with log MR equal to or higher than the antimode were classified as poor metabolizers (PMs) and those with values less than antimode were categorized as extensive metabolizers (EMs).
Results: Data from a total of 149 participants were evaluated and the median (range) of MR was 0.25 (0.03-3.01). The polynomial equation obtained in probit analysis gave an antimode for MR of 1.39. Five (3.36%) participants were PMs and 144 (96.64%) were found to be EMs. One participant had reported mild drowsiness 2 h postdose that subsided spontaneously without any intervention.
Conclusion: The prevalence of CYP2D6 polymorphism in Western Indian population is low (3.36%) and is similar to other populations.
Nithya J Gogtay
Department of Clinical Pharmacology, Seth G S Medical College and KEM Hospital, Mumbai, Maharashtra
|How to cite this article:|
Gogtay NJ, Mali NB, Iyer K, Kadam PP, Sridharan K, Shrimal D, Thatte UM. Evaluation of cytochrome P450 2D6 phenotyping in healthy adult Western Indians.Indian J Pharmacol 2014;46:266-269
|How to cite this URL:|
Gogtay NJ, Mali NB, Iyer K, Kadam PP, Sridharan K, Shrimal D, Thatte UM. Evaluation of cytochrome P450 2D6 phenotyping in healthy adult Western Indians. Indian J Pharmacol [serial online] 2014 [cited 2021 May 7 ];46:266-269
Available from: https://www.ijp-online.com/article.asp?issn=0253-7613;year=2014;volume=46;issue=3;spage=266;epage=269;aulast=Gogtay;type=0