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Year : 2011  |  Volume : 43  |  Issue : 5  |  Page : 551--556

Pharmacological investigations of Punica granatum in glycerol-induced acute renal failure in rats

Amrit Pal Singh, Amteshwar Jaggi Singh, Nirmal Singh 
 Department of Pharmaceutical Sciences and Drug Research, Punjabi University, Patiala 147 002, India

Correspondence Address:
Nirmal Singh
Department of Pharmaceutical Sciences and Drug Research, Punjabi University, Patiala 147 002
India

Objective : The present study was designed to investigate the ameliorative potential and possible mechanism of hydroalcoholic extract of flowers of P. granatum in glycerol-induced acute renal failure (ARF) in rats. Materials and Methods : The rats were subjected to rhabdomyolytic ARF by single intramuscular injection of hypertonic glycerol (50% v/v; 8 ml/kg) and the animals were sacrificed after 24 hours of glycerol injection. The plasma creatinine, blood urea nitrogen, creatinine clearance, and histopathological studies were performed to assess the degree of renal injury. Results : Pretreatment with hydroalcoholic extract of flowers of P. granatum (125 and 250 mg/kg p.o. twice daily for 3 days) significantly attenuated hypertonic glycerol-induced renal dysfunction in a dose-dependent manner. BADGE (Bisphenol-A-diglycidyl ether) (30 mg/kg), a peroxisome proliferator-activated receptor (PPAR)-γ antagonist, and N(omega)-nitro-l-arginine-methyl ester (L-NAME) (10, 20, and 40 mg/kg), nitric oxide synthase inhibitor, were employed to explore the mechanism of renoprotective effects of Punica granatum. Administration of BADGE (30 mg/kg) and L-NAME (40 mg/kg) abolished the beneficial effects of P. granatum in glycerol-induced renal dysfunction. Conclusion : Hydroalcoholic extract of flowers of P. granatum has ameliorative potential in attenuating myoglobinuric renal failure and its renoprotective effects involve activation of PPAR-γ and nitric oxide-dependent signaling pathway.


How to cite this article:
Singh AP, Singh AJ, Singh N. Pharmacological investigations of Punica granatum in glycerol-induced acute renal failure in rats.Indian J Pharmacol 2011;43:551-556


How to cite this URL:
Singh AP, Singh AJ, Singh N. Pharmacological investigations of Punica granatum in glycerol-induced acute renal failure in rats. Indian J Pharmacol [serial online] 2011 [cited 2022 Oct 5 ];43:551-556
Available from: https://www.ijp-online.com/article.asp?issn=0253-7613;year=2011;volume=43;issue=5;spage=551;epage=556;aulast=Singh;type=0