Indian Journal of Pharmacology Home 

RESEARCH ARTICLE
[View FULLTEXT] [Download PDF]
Year : 2011  |  Volume : 43  |  Issue : 2  |  Page : 187--191

The effect of mitoxantrone as an anticancer drug on hepatocytes nuclei and chromatin: Selective release of histone proteins

Zahra Hajihassan, Azra Rabbani-Chadegani 
 Department of Biochemistry, Institute of Biochemistry and Biophysics, University of Tehran, Tehran, Iran

Correspondence Address:
Azra Rabbani-Chadegani
Department of Biochemistry, Institute of Biochemistry and Biophysics, University of Tehran, Tehran
Iran

Objectives : Mitoxantrone is an anticancer drug widely used in the treatment of various cancers. In the present study the effect of mitoxantrone on chromatin proteins of intact hepatocytes nuclei was investigated and compared with soluble chromatin. Materials and Methods : UV/Vis spectroscopy, SDS polyacrylamide gel electrophoresis, and western bolting were used. Results : The results show that exposure of intact nuclei to various concentrations of mitoxantrone resulted in the release of histone H1 family proteins, H1 and H1°, in a dose-dependent manner but not core histones and high mobility group proteins. Western blot analysis using antiserum against histones H1 and H1° revealed cross-reactivity and confirmed the result. Spectroscopy results showed that mitoxantrone binds to nuclear components and reduces the absorbances at 608 and 400 nm. The binding isotherms revealed cooperative binding with one binding site. Conclusion : From the results it is suggested that mitoxantrone binds to intact nuclei and chromatin with different affinities and linker DNA can be considered as a main binding site for mitoxantrone at the nuclei level.


How to cite this article:
Hajihassan Z, Rabbani-Chadegani A. The effect of mitoxantrone as an anticancer drug on hepatocytes nuclei and chromatin: Selective release of histone proteins.Indian J Pharmacol 2011;43:187-191


How to cite this URL:
Hajihassan Z, Rabbani-Chadegani A. The effect of mitoxantrone as an anticancer drug on hepatocytes nuclei and chromatin: Selective release of histone proteins. Indian J Pharmacol [serial online] 2011 [cited 2021 May 6 ];43:187-191
Available from: https://www.ijp-online.com/article.asp?issn=0253-7613;year=2011;volume=43;issue=2;spage=187;epage=191;aulast=Hajihassan;type=0