Anxiolytic activity of Nymphaea alba Linn. in mice as experimental models of anxiety
BS Thippeswamy1, Brijesh Mishra1, VP Veerapur2, Gourav Gupta1
1 Department of Pharmacology, Sree Siddaganga College of Pharmacy, Tumkur - 572 102, Karnataka, India
2 Quality Assurance, Sree Siddaganga College of Pharmacy, Tumkur - 572 102, Karnataka, India
Objective : The aim of the present work was to evaluate the anxiolytic effect of an ethanolic extract of Nymphaea alba Linn. in mice.
Materials and Methods : The elevated plus maze test (EPMT), light and dark test (L and DT) and open field test (OFT) were used to assess the anxiolytic activity of the ethanolic extract of N. alba Linn. in mice. In addition, aggressive behavior and motor coordination was also assessed by foot shock induced aggression test (FSIAT) and rota rod test (RRT). Diazepam 1 mg/kg served as a standard anxiolytic drug, administered orally.
Results : The ethanolic extract of N. alba (100 and 200 mg/kg, p.o.) significantly increased the percentage of time spent and number of entries in open arm in EPMT. In L and DT, the extract produced significant increase in time spent, number of crossing and decrease in the duration of immobility in light box. In OFT, the extract showed significant increase in number of rearings, assisted rearings and number of square crossed, all of which are demonstrations of exploratory behavior. In FSIAT, N. alba extract attenuated aggressive behavior related to anxiolytic activity, such as number of vocalization, leaps, rearing, biting/attacks and facing each other in paired mice. Furthermore, the extract produced skeletal muscle relaxant effect assessed by RRT.
Conclusion : The results of the present study suggest that an ethanolic extract of N. alba may possess anxiolytic activity and provide a scientific evidence for its traditional claim.
B S Thippeswamy
Department of Pharmacology, Sree Siddaganga College of Pharmacy, Tumkur - 572 102, Karnataka
|How to cite this article:|
Thippeswamy B S, Mishra B, Veerapur V P, Gupta G. Anxiolytic activity of Nymphaea alba Linn. in mice as experimental models of anxiety.Indian J Pharmacol 2011;43:50-55
|How to cite this URL:|
Thippeswamy B S, Mishra B, Veerapur V P, Gupta G. Anxiolytic activity of Nymphaea alba Linn. in mice as experimental models of anxiety. Indian J Pharmacol [serial online] 2011 [cited 2021 Apr 21 ];43:50-55
Available from: https://www.ijp-online.com/article.asp?issn=0253-7613;year=2011;volume=43;issue=1;spage=50;epage=55;aulast=Thippeswamy;type=0