Evaluation of Cassia occidentalis for in vitro cytotoxicity against human cancer cell lines and antibacterial activity
Madhulika Bhagat, Ajit Kumar Saxena
School of Biotechnology, University of Jammu, Jammu, India
Objective : To evaluate the in vitro cytotoxicity and antibacterial properties of Cassia occidentalis (whole plant) via alcoholic, hydro-alcoholic, and aqueous extracts against eight human cancer cell lines from six different tissues and four bacterial strains.
Material and Methods : In vitro cytotoxicity against the human cancer cells, cultured for 48h in presence of different concentrations C. occidentalis extracts and percentage of cell viability, was evaluated using the sulforhodamine-B (SRB) assay. The antibacterial activity was performed using the standard protocol against bacterial strains.
Results : It was observed that aqueous extract of C. occidentalis (whole plant) had more potential than hydro-alcoholic and alcoholic extracts against HCT-15, SW-620, PC-3, MCF-7, SiHa, and OVCAR-5 human cancer cell lines at 100, 30, and 10 μg/ml in a dose-dependent manner. The hydro-alcoholic extract showed potential against Bacillus subtillis.
Conclusion : The plant can be explored for the possible development of lead molecules for drug discovery.
School of Biotechnology, University of Jammu, Jammu
|How to cite this article:|
Bhagat M, Saxena AK. Evaluation of Cassia occidentalis for in vitro cytotoxicity against human cancer cell lines and antibacterial activity.Indian J Pharmacol 2010;42:234-237
|How to cite this URL:|
Bhagat M, Saxena AK. Evaluation of Cassia occidentalis for in vitro cytotoxicity against human cancer cell lines and antibacterial activity. Indian J Pharmacol [serial online] 2010 [cited 2021 Oct 18 ];42:234-237
Available from: https://www.ijp-online.com/article.asp?issn=0253-7613;year=2010;volume=42;issue=4;spage=234;epage=237;aulast=Bhagat;type=0