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Year : 2010  |  Volume : 42  |  Issue : 4  |  Page : 208--213

Renal effects of Mammea africana Sabine (Guttiferae) stem bark methanol/methylene chloride extract on L-NAME hypertensive rats

Elvine Pami Nguelefack-Mbuyo1, Theophile Dimo2, Telesphore Benoit Nguelefack4, Alain Bertrand Dongmo3, Pierre Kamtchouing2, Albert Kamanyi5 
1 Laboratory of Animal Physiology, Department of Animal Biology and Physiology, University of Yaounde I, P.O.Box 812 Yaounde; Laboratory of Animal Physiology and Phytopharmacology, Department of Animal Biology, University of Dschang, P.O.Box 67 Dschang, Cameroon
2 Laboratory of Animal Physiology, Department of Animal Biology and Physiology, University of Yaounde I, P.O.Box 812 Yaounde, Cameroon
3 Department of Animal Biology and Physiology, University of Douala, BP 24157 Douala, Cameroon

Correspondence Address:
Albert Kamanyi
Laboratory of Animal Physiology and Phytopharmacology, Department of Animal Biology, University of Dschang, P.O.Box 67 Dschang
Cameroon

Objective : The present study aims at evaluating the effects of methanol/methylene chloride extract of the stem bark of Mammea africana on the renal function of L-NAME treated rats. Material and Methods : Normotensive male Wistar rats were divided into five groups respectively treated with distilled water, L-NAME (40 mg/kg/day), L-NAME + L-arginine (100 mg/kg/day), L-NAME + captopril (20 mg/kg/day) or L-NAME + M. africana extract (200 mg/kg/day) for 30 days. Systolic blood pressure was measured before and at the end of treatment. Body weight was measured at the end of each week. Urine was collected 6 and 24 h after the first administration and further on day 15 and 30 of treatment for creatinine, sodium and potassium quantification, while plasma was collected at the end of treatment for the creatinine assay. ANOVA two way followed by Bonferonni or one way followed by Tukey were used for statistical analysis. Results : M. africana successfully prevented the rise in blood pressure and the acute natriuresis and diuresis induced by L-NAME. When given chronically, the extract produced a sustained antinatriuretic effect, a non-significant increase in urine excretion and reduced the glomerular hyperfiltration induced by L-NAME. Conclusions : The above results suggest that the methanol/methylene chloride extract of the stem bark of M. africana may protect kidney against renal dysfunction and further demonstrate that its antihypertensive effect does not depend on a diuretic or natriuretic activity.


How to cite this article:
Nguelefack-Mbuyo EP, Dimo T, Nguelefack TB, Dongmo AB, Kamtchouing P, Kamanyi A. Renal effects of Mammea africana Sabine (Guttiferae) stem bark methanol/methylene chloride extract on L-NAME hypertensive rats.Indian J Pharmacol 2010;42:208-213


How to cite this URL:
Nguelefack-Mbuyo EP, Dimo T, Nguelefack TB, Dongmo AB, Kamtchouing P, Kamanyi A. Renal effects of Mammea africana Sabine (Guttiferae) stem bark methanol/methylene chloride extract on L-NAME hypertensive rats. Indian J Pharmacol [serial online] 2010 [cited 2021 Oct 20 ];42:208-213
Available from: https://www.ijp-online.com/article.asp?issn=0253-7613;year=2010;volume=42;issue=4;spage=208;epage=213;aulast=Nguelefack-Mbuyo;type=0