Expression of TNF-α and RANTES in drug-induced human gingival overgrowth
Tamilselvan Subramani1, Loganathan Dhanaraj2, Kamatchiammal Senthilkumar2, Soundararajan Periasamy3, Georgi Abraham3, Suresh Rao1
1 Department of Periodontics, Sri Ramachandra Dental College and Hospital, Porur, Chennai - 600 116, India
2 NEERI, Council of Scientific and Industrial Research, Taramani, Chennai - 600 113, India
3 Department of Nephrology, Sri Ramachandra Medical College and Research Institute, Sri Ramachandra University, Porur, Chennai - 600 116, India
Objectives : Regulated on activation, normal T cell expressed and secreted (RANTES) is a chemokine that is produced by fibroblasts, lymphoid and epithelial cells of the mucosa in response to various external stimuli. RANTES expression has been demonstrated in a variety of diseases characterized by inflammation, including asthma, transplantation-associated accelerated atherosclerosis, endometriosis and fibrosis. RANTES mRNA is quickly up-regulated by tumor necrosis factor (TNF)-α stimulation. Cyclosporine A (CsA) is widely used in organ transplant patients, often causing various side-effects including gingival overgrowth, which is fibrotic in nature. This study was carried out to assess the mRNA expression of TNF-α and RANTES in healthy individual, chronic periodontitis and CsA-induced gingival overgrowth tissues.
Materials and Methods : Gingival tissue samples were collected from chronic periodontitis, CsA-induced gingival overgrowth patients and healthy individuals. Total RNA was isolated and reverse transcription polymerase chain reaction (RT-PCR) was performed for TNF-α and RANTES expression.
Results : The results suggest that CsA-induced gingival overgrowth tissues expressed significantly increased TNF-α and RANTES compared to control and chronic periodontitis.
Conclusion : The findings of the present study suggest that CsA can modify the expression of TNF-α and RANTES in drug-induced human gingival overgrowth.
Department of Periodontics, Sri Ramachandra Dental College and Hospital, Porur, Chennai - 600 116
|How to cite this article:|
Subramani T, Dhanaraj L, Senthilkumar K, Periasamy S, Abraham G, Rao S. Expression of TNF-α and RANTES in drug-induced human gingival overgrowth.Indian J Pharmacol 2010;42:174-177
|How to cite this URL:|
Subramani T, Dhanaraj L, Senthilkumar K, Periasamy S, Abraham G, Rao S. Expression of TNF-α and RANTES in drug-induced human gingival overgrowth. Indian J Pharmacol [serial online] 2010 [cited 2021 Sep 20 ];42:174-177
Available from: https://www.ijp-online.com/article.asp?issn=0253-7613;year=2010;volume=42;issue=3;spage=174;epage=177;aulast=Subramani;type=0