Calcium channel blockers reduce inflammatory edema in the rat: Involvement of the hypothalamus-pituitary-adrenal axis
M Khaksari1, SE Mahani2, M Mahmoodi3
1 Department of Physiology and Pharmacology, Faculty of Medicine, Kerman, Iran
2 Department of Physiology, Jahrom School of Medicine, Jahrom, Iran
3 Department of Biochemistry, Faculty of Medicine, Rafsanjan, Iran
OBJECTIVE: To evaluate the role of calcium channel blockers and their mechanisms of action on acute inflammation of rat paw.
MATERIAL AND METHODS: The study was conducted using carrageenan-induced rat paw inflammation model. Two different doses of nifedipine and verapamil (25 and 400 µg/kg, i.p.) were used. Edema was assessed by calculating the volume changes and by extravasation of Evans blue dye.
RESULTS: Nifedipine reduced edema dose-dependently, whereas verapamil was effective only at low dose. Adrenalectomy prevented the effect of nifedipine and verapamil. With low dose of nifedipine 66% of antiinflammatory effect was observed. Pretreatment with -helical corticotropin releasing factor (CRF 9-41), a corticotropin-releasing hormone (CRH) receptor antagonist, had the same effect as that of adrenalectomy for either doses of verapamil, but only the effect of low-dose nifedipine was prevented completely.
CONCLUSION: Our data suggest that verapamil and nifedipine exerts a potent antiinflammatory action possibly through pituitary adrenocortical activation.
Department of Physiology and Pharmacology, Faculty of Medicine, Kerman
|How to cite this article:|
Khaksari M, Mahani S E, Mahmoodi M. Calcium channel blockers reduce inflammatory edema in the rat: Involvement of the hypothalamus-pituitary-adrenal axis.Indian J Pharmacol 2004;36:351-354
|How to cite this URL:|
Khaksari M, Mahani S E, Mahmoodi M. Calcium channel blockers reduce inflammatory edema in the rat: Involvement of the hypothalamus-pituitary-adrenal axis. Indian J Pharmacol [serial online] 2004 [cited 2023 Mar 27 ];36:351-354
Available from: https://www.ijp-online.com/article.asp?issn=0253-7613;year=2004;volume=36;issue=6;spage=351;epage=354;aulast=Khaksari;type=0