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|Year : 1999 | Volume
| Issue : 6 | Page : 440--441
Bioassay of acetylcholine with horizontal strips of rat fundus
Sa SB De1, Sa SB De2, VG Dhume3
Objective: To find out the suitability of horizontal strips of rat fundus for bioassay of acetylcholine (ACh).
Methods: Dose response curves to ACh were obtained using a horizontal strip of rat fundus suspended in Tyrode solution. The effects of histamine (H) and 5-hydroxytryptamine (5-HT) were also studied.
Results: Horizontal strips of rat fundus were stable over a long period of time (more than 6 hrs) showing reproducible responses to Ach with good discrimination between doses. It did not respond to H and 5-HT. Thus it was specific to ACh, the sensitivity being 10 -8 g/ml.
Conclusion: The modified preparation of rat fundus described by us is simple and satisfies the prerequisites for the bioassay of ACh.
Sa SB De
|How to cite this article:|
De SS, De SS, Dhume V G. Bioassay of acetylcholine with horizontal strips of rat fundus.Indian J Pharmacol 1999;31:440-441
|How to cite this URL:|
De SS, De SS, Dhume V G. Bioassay of acetylcholine with horizontal strips of rat fundus. Indian J Pharmacol [serial online] 1999 [cited 2021 Nov 28 ];31:440-441
Available from: https://www.ijp-online.com/article.asp?issn=0253-7613;year=1999;volume=31;issue=6;spage=440;epage=441;aulast=De;type=0