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Year : 1999  |  Volume : 31  |  Issue : 3  |  Page : 204--213

A mitochondrial perspective on ischaemic brain damage

PD Chowdhury1, M Ray2, MP Dubey3 

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P D Chowdhury

Objective: To investigate the role of mtNOS in cerebral ischaemia in Mongolian gerbils. Methods: Mitochondria isolated after 5 min of ischaemia and after 48 and 72 hrs of restitution of blood flow was used. Animals were pretreated with fast Na + channel blocks (TTX) and NOS inhibitors (L-NAME, 7-NI). Following estimations were made: (i) respiratory main complexes (ii) NAD(P)H/NAD(P) levels (iii) mitochondrial membrane potential and (iv) DNA pattern. Results: LDH estimated in the venous outflow was elevated. Respiratory main complexes I, II and III except IV were altered in ischaemic group. NAD(P)H/NAD(P) levels were elevated, significant decrease with TTX in all groups was observed. Ischaemia dissipated mitochondrial membrane potential. This was reverted close to normal values by TTX, 7-NI and L-NAME in the order of their efficacy. No significant change in DNA pattern was observed. Conclusion: Ischaemia induced pathological changes at the mitochondria level are presented by TTX and L-NAME. They may find possible use for therapeutic interventions in neurodegenerative disorders.

How to cite this article:
Chowdhury P D, Ray M, Dubey M P. A mitochondrial perspective on ischaemic brain damage.Indian J Pharmacol 1999;31:204-213

How to cite this URL:
Chowdhury P D, Ray M, Dubey M P. A mitochondrial perspective on ischaemic brain damage. Indian J Pharmacol [serial online] 1999 [cited 2022 Aug 14 ];31:204-213
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