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Year : 1998  |  Volume : 30  |  Issue : 4  |  Page : 263--266

Simple spectrofluorometric and microbiological assay methods for the estimation of ofloxacin in biological fluids

Gurumurthy Prema1, Ramachandran Geetha2, AK Hemanthkumar3, P Venkatesan4, V Chandrasekaran5, PR Narayanan6 
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Correspondence Address:
Gurumurthy Prema


Objective: To evolve simple methods for the assay of ofloxacin in biological fluids. Methods: Simple methods for the estimation of ofloxacin in plasma, saliva and urine employing micro-biological assay using plate diffusion technique and by fluorimetric method based on the measurement of native fluorescence emitted by ofloxacin, have been described. Results: The recovery of ofloxacin from all the three biologial fluids was 93-98% and the sensitivity was 0.5 g/ml on all 5 different occasions by both the methods. Anti-TB drugs viz., rifampicin, ethambutol, isoniazid and pyrazinamide and also anti-leprosy drugs viz., dapsone and clofazimine at concentrations of 10 and 20 g/ml did not interfere with the estimation of ofloxacin by either method. Ofloxacin is stable in biological fluids for a period of at least 8 days at -20 C. Conclusion: Both the methods described are simple, involve very few steps and do not need either costly chemicals or sophisticated equipments.


How to cite this article:
Prema G, Geetha R, Hemanthkumar A K, Venkatesan P, Chandrasekaran V, Narayanan P R. Simple spectrofluorometric and microbiological assay methods for the estimation of ofloxacin in biological fluids.Indian J Pharmacol 1998;30:263-266


How to cite this URL:
Prema G, Geetha R, Hemanthkumar A K, Venkatesan P, Chandrasekaran V, Narayanan P R. Simple spectrofluorometric and microbiological assay methods for the estimation of ofloxacin in biological fluids. Indian J Pharmacol [serial online] 1998 [cited 2021 Dec 9 ];30:263-266
Available from: https://www.ijp-online.com/article.asp?issn=0253-7613;year=1998;volume=30;issue=4;spage=263;epage=266;aulast=Prema;type=0