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Year : 1983  |  Volume : 15  |  Issue : 4  |  Page : 255--260

Interaction of 2H arecoline with SH group and other biomolecules

SV Bhide1, NM Shivapurkar2 
1 
2 

Correspondence Address:
S V Bhide


: In an attempt to understand the mechanism of action of arecoline at molecular level, interaction of 3H arecoline with DNA, RNA, Protein in glutathione was studied in Swiss mice. Six week old Swiss strain male mice were injected (ip) with 3H arecoline (1 ęci/g body wt). Animals were killed at different time intervals (10 min, 3 h, 18 h 24 h, 7 days) after the injection. DNA, RNA, protein and glutathione were isolated by standard methods and radioactivity was measured from these fractions. Among all these fractions maximum specific activity was observed in glutathione fraction and next to it in DNA fraction Significantly high radioactivity was observed in all the fractions under study at 10 minutes indicating possibility of interaction with intact arecoline. Further, pretreatment of sulfhydryl reaction reagents decreased the specific activity of protein in mice injected with 3H arecoline.


How to cite this article:
Bhide S V, Shivapurkar N M. Interaction of 2H arecoline with SH group and other biomolecules.Indian J Pharmacol 1983;15:255-260


How to cite this URL:
Bhide S V, Shivapurkar N M. Interaction of 2H arecoline with SH group and other biomolecules. Indian J Pharmacol [serial online] 1983 [cited 2021 Nov 27 ];15:255-260
Available from: https://www.ijp-online.com/article.asp?issn=0253-7613;year=1983;volume=15;issue=4;spage=255;epage=260;aulast=Bhide;type=0