| CLINICAL RESEARCH ARTICLES |
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| Year : 2022 | Volume
: 54
| Issue : 3 | Page : 171-176 |
High-level gentamicin resistance mediated by Aac(6′)-Ie-aph(2”)-Ia gene in Enterococcus species isolated from clinical samples in Northern India
Ayan Kumar Das1, Mridu Dudeja1, Sunil Kohli2, Pratima Ray3, Shyamasree Nandy1
1 Department of Microbiology, Hamdard Institute of Medical Sciences and Research, Jamia Hamdard University, New Delhi, India
2 Medicine, Hamdard Institute of Medical Sciences and Research, Jamia Hamdard University, New Delhi, India
3 Department of Biotechnology, School of Chemical and Life Sciences, Jamia Hamdard University, New Delhi, India
Correspondence Address:
Dr. Ayan Kumar Das
Department of Microbiology, Hamdard Institute of Medical Sciences and Research, Jamia Hamdard University, New Delhi - 110 062
India
 Source of Support: None, Conflict of Interest: None  | Check |
DOI: 10.4103/ijp.IJP_41_20
CONTEXT: Enterococci are known to cause life-threatening infections which are difficult to treat as the organism harbors innate resistance to many antibiotics and can amass resistance toward many others through plasmid-mediated genetic exchange.
AIMS: The study evaluates the drug susceptibility profile of various Enterococcus species isolated from various patient specimens submitted for bacteriological analysis and check the incidence of aac(6′) Ie-aph(2”) Ia gene.
SETTING AND DESIGN: This in vitro cross-sectional study was executed at bacteriology laboratory of a 470 bedded hospital in New Delhi.
MATERIALS AND METHODS: Drug susceptibility testing was carried out on enterococcal isolates. High-level gentamicin-resistant (HLGR) isolates detected by micro broth dilution assay were then subjected to molecular detection of aac(6′) Ie-aph(2”) Ia gene.
STATISTICAL ANALYSIS USED: The level of significance was established by Chi-square test.
RESULTS: Among the 182 enterococcal stains detected, 76.9% were Enterococcus faecalis and 20.3% were Enterococcus faecium. 12.08% strains were vancomycin resistant. 39% expressed resistance toward high-level gentamicin (HLG) and this finding was significantly higher in E. faecium than E. faecalis. HLGR strains expressed a higher degree of resistance to other drugs in contrast to non-HLGR isolates. In 67 out of 71 HLGR isolates the bifunctional gene was detected.
CONCLUSION: Considerable presence of HLG and vancomycin resistance in the clinical isolates is alarming and should be taken seriously. The study shows high dissemination of aac(6′)-Ie-aph(2”)-Ia gene among Enterococci isolated from the region.
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