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| RESEARCH ARTICLE
|Year : 2022 | Volume
| Issue : 1 | Page : 33-40
Studies on biochemical, oxidative and genotoxicity alterations following vas blockage with reversible inhibition of sperm under guidance and reversal in rats
Abdul S Ansari1, Mubarik Hussain2, Sadi Rehan Khan1, Ayesha Badar1, Barkha Khilwani1, Nirmal Kumar Lohiya1
1 Department of Zoology, Centre for Advanced Studies, University of Rajasthan, Sitapura, Jaipur, Rajasthan, India
2 Department of Zoology, S. S. Jain Subodh College of Global Excellence, Sitapura, Jaipur, Rajasthan, India
CONTEXT: Vas obstruction with reversible inhibition of sperm under guidance (RISUG) for contraception and its reversal, may cause oxidative stress or inimical effects on male reproductive functions.
OBJECTIVE: To evaluate the biochemical and genotoxicity at the level of reactive oxygen species (ROS) following vas occlusion with RISUG and its reversal by Dimethyl sulphoxide (DMSO) and 5% NaHCO3 in Wistar albino rats.
SETTINGS AND DESIGN: Animals were divided into seven groups (n = 10), namely sham-operated control, short-term vas occlusion with RISUG for 90 days, reversal with DMSO and 5% NaHCO3, long-term vas occlusion with RISUG for 360 days, reversal with DMSO and 5% NaHCO3.
MATERIALS AND METHODS: Biochemical markers in reproductive tissues, hematology, serum biochemistry, serum electrolytes and ROS measuring indicators, e.g., lipid peroxidation, superoxide dismutase, catalase, glutathione peroxidase, and glutathione S-transferase were examined.
STATISTICAL ANALYSIS USED: One-way analysis of variance test was performed for analyses of data obtained in this study using the SPSS 10.0 software (SPSS Inc., Chicago, IL, USA).
RESULTS: The tissue and clinical chemistry did not show appreciable alterations in RISUG injected and reversal Groups (II-VII) as compared to sham control. The genotoxicity and various ROS markers fluctuated within control limits following short- and long-term vas occlusion and reversal.
CONCLUSIONS: The study suggested that the reversal procedures, following RISUG contraception, were not associated with any kind of toxicological manifestations.
Prof. Abdul S Ansari
Department of Zoology, Centre for Advanced Studies, University of Rajasthan, Jaipur - 302 004, Rajasthan
Source of Support: None, Conflict of Interest: None
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