| RESEARCH ARTICLE |
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| Year : 2021 | Volume
: 53
| Issue : 1 | Page : 13-18 |
Determination of in vitro human whole blood-to-plasma ratio of THJ-018 utilizing gas chromatography–Mass spectrometry
Sachil Kumar1, Remal Nahar Khleel Alkhibery1, Abdulsallam Bakdash1, Mohamed Sultan Mohamed Abdelhady2
1 Department of Forensic Sciences, College of Criminal Justice, Naif Arab University for Security Sciences, Riyadh, Saudi Arabia
2 Riyadh Poisons Control & Medical Forensic Chemistry Center, Kaab Ibn Al Harith, Ar Rawdah, Riyadh, Saudi Arabia; Egyptian Drug Authority, National Organizations for Drug Control and Research (NODCAR), Giza, Egypt
Correspondence Address:
Dr. Sachil Kumar
Assistant Professor, Department of Forensic Sciences, College of Criminal Justice, Naif Arab University for Security Sciences, Riyadh
Saudi Arabia
 Source of Support: None, Conflict of Interest: None  | Check |
DOI: 10.4103/ijp.IJP_668_19
OBJECTIVES: This study was aimed to determine in vitro human whole blood-to-plasma ratio (KWB/P) of THJ-018 by gas chromatography/mass spectrometry (GC/MS).
MATERIALS AND METHODS: The samples (human blood) were sprayed with THJ-018 and an internal standard and extracted using solid-phase extraction. THJ-018 was determined in the final extracts by GC/MS.
RESULTS: The value for KWB/P was 1.56 (1.38–1.81), and red blood cell partitioning was 1.01 (1.01–1.02). The distribution of THJ-018 between whole blood and plasma was observed to be affected by temperature.
CONCLUSION: The data analysis supports the proposition that the ratio of the plasma to whole blood concentrations (1.56) is a suitable parameter characterizing THJ-018 distribution in whole blood. For toxicological analysis, it would be best to refrain from converting any drug concentration measured in whole blood to that anticipated in plasma or serum; however, toxic and therapeutic concentrations should be determined for the individual specimens collected.
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