| RESEARCH ARTICLE
|Year : 2021 | Volume
| Issue : 1 | Page : 13-18
Determination of in vitro human whole blood-to-plasma ratio of THJ-018 utilizing gas chromatography–Mass spectrometry
Sachil Kumar1, Remal Nahar Khleel Alkhibery1, Abdulsallam Bakdash1, Mohamed Sultan Mohamed Abdelhady2
1 Department of Forensic Sciences, College of Criminal Justice, Naif Arab University for Security Sciences, Riyadh, Saudi Arabia
2 Riyadh Poisons Control & Medical Forensic Chemistry Center, Kaab Ibn Al Harith, Ar Rawdah, Riyadh, Saudi Arabia; Egyptian Drug Authority, National Organizations for Drug Control and Research (NODCAR), Giza, Egypt
OBJECTIVES: This study was aimed to determine in vitro human whole blood-to-plasma ratio (KWB/P) of THJ-018 by gas chromatography/mass spectrometry (GC/MS).
MATERIALS AND METHODS: The samples (human blood) were sprayed with THJ-018 and an internal standard and extracted using solid-phase extraction. THJ-018 was determined in the final extracts by GC/MS.
RESULTS: The value for KWB/P was 1.56 (1.38–1.81), and red blood cell partitioning was 1.01 (1.01–1.02). The distribution of THJ-018 between whole blood and plasma was observed to be affected by temperature.
CONCLUSION: The data analysis supports the proposition that the ratio of the plasma to whole blood concentrations (1.56) is a suitable parameter characterizing THJ-018 distribution in whole blood. For toxicological analysis, it would be best to refrain from converting any drug concentration measured in whole blood to that anticipated in plasma or serum; however, toxic and therapeutic concentrations should be determined for the individual specimens collected.
Dr. Sachil Kumar
Assistant Professor, Department of Forensic Sciences, College of Criminal Justice, Naif Arab University for Security Sciences, Riyadh
Source of Support: None, Conflict of Interest: None
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