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| RESEARCH ARTICLE
|Year : 2016 | Volume
| Issue : 1 | Page : 32-36
The effect of metamizole on ischemia/reperfusion injury in the rat ovary: An analysis of biochemistry, molecular gene expression, and histopathology
Serkan Kumbasar1, Suleyman Salman2, Ragip Atakan Al3, Cengiz Ozturk4, Oguzhan Yarali5, Hamit Hakan Alp6, Durdu Altuner7, Bahadir Suleyman7
1 Department of Obstetrics and Gynecology, School of Medicine, Sakarya University, Sakarya, Turkey
2 Department of Obstetrics and Gynecology, Gaziosmanpasa Taksim Education and Research Hospital, Istanbul, Turkey
3 Department of Obstetrics and Gynecology, Faculty of Medicine, Ataturk University, Erzurum, Turkey
4 Department of Anatomy, Erzurum Region Education and Research Hospital, Erzurum, Turkey
5 Department of Medical Genetics, Istanbul Medeniyet University Goztepe Training and Research Hospital, Istanbul, Turkey
6 Department of Biochemistry, Faculty of Medicine, 100. Yil University, Van, Turkey
7 Department of Pharmacology, Faculty of Medicine, Erzincan University, Erzincan, Turkey
Objectives: In this study, we investigated the effect of metamizole on ischemia/reperfusion (I/R) injury an analysis of biochemistry, molecular gene expression, and histopathology in the rat ovary of female albino Wistar rats.
Materials and Methods: Animals were divided into four groups; control group with induced ischemia-reperfusion (IRC), ischemia-reperfusion +100 mg/kg metamizole sodium (MS) (IRM-100), ischemia-reperfusion +200 mg/kg MS (IRM-200), and healthy group applied sham operation (SG).
Results: Myeloperoxidase (MPO) activity and gene expression increased significantly in IRC and IRM-100 group rat ovarian tissue compared with the SG group (P < 0.0001). However, MPO activity and gene expression in IRM-200 group ovarian tissue decreased significantly compared with the IRC and IRM-100 groups (P < 0.0001). Histopathologically, pronounced congestion, dilated vessels, hemorrhage, edema, degenerative cells, and neutrophil migration and adhesion to the endothelium were observed in the IRC and IRM-100 group ovarian tissues. A small number of congested dilated vessels, mild congestion, and edema were observed in the IRM-200 group, but no neutrophil migration and adhesion to the endothelium or degenerative cells.
Conclusions: At 200 mg/kg dose metamizole prevented ovarian injury induced with I/R. This data show that metamizole can be used in the ovarian I/R injury treatment.
Department of Pharmacology, Faculty of Medicine, Erzincan University, Erzincan
Source of Support: None, Conflict of Interest: None
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