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Year : 2015  |  Volume : 47  |  Issue : 4  |  Page : 447-450

Anti-inflammatory activity of Nerium indicum by inhibition of prostaglandin E2 in murine splenic lymphocytes

Department of Zoology, Cellular Immunology Laboratory, University of North Bengal, Siliguri, West Bengal, India

Correspondence Address:
Prof. Tapas Kumar Chaudhuri
Department of Zoology, Cellular Immunology Laboratory, University of North Bengal, Siliguri, West Bengal
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Source of Support: None, Conflict of Interest: None

DOI: 10.4103/0253-7613.161275

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Objective: Nerium indicum Mill (syn. N. oleander L. and N. odorum Aiton; family: Apocynaceae) is a medicinal plant, used in the treatment of diverse ailments including various chronic inflammatory diseases in traditional medicine. We have previously demonstrated the immunomodulatory activity of a bioactive fraction of Nerium indicum leaf (NILE) by studying up-regulation of interleukin-2 (IL-2), IL-10, interferon-gamma and down regulation of IL-4, tumor necrosis factor-alpha (TNF-α), nitric oxide, cyclooxygenase-1 (COX-1) and COX-2 activities. Therefore, this study aimed to confirm the anti-inflammatory activity of NILE by inhibition of prostaglandin E 2 (PGE 2 ) activity in murine splenic lymphocytes in vitro. Materials and Methods: Murine lymphocytes were isolated from spleen and stimulated with 5 ΅g/mL concanavalin A in RPMI-1640, supplemented with 50 U/mL penicillin, 50 U/mL streptomycin, 50 U/mL nystatin and 10% fetal bovine serum. Different concentrations (0-80 μg/mL) of NILE were added and the cells were cultured for 48 h. The culture supernatants were thereafter collected by centrifugation and assayed for expression of PGE 2 level. The data were analyzed statistically. Results: The results demonstrated a 2.26-fold inhibition of PGE 2 level at 80 μg/mL of NILE. Half maximum inhibitory concentration (IC 50 ) was calculated to be 44.95 ± 0.45 ΅g/mL. Linear correlation analysis of the dose-dependent PGE 2 inhibition with other pro- and anti-inflammatory mediators demonstrated high inter-correlation between the parameters. Conclusions: Thus, the present study remains in accordance with our previous report and confirms the anti-inflammatory claim of N. indicum, mentioned in the traditional medicine.


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