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LETTER TO THE EDITOR
Year : 2014  |  Volume : 46  |  Issue : 3  |  Page : 350-351
 

In vitro study on α-amylase inhibitory activity of an Ayurvedic medicinal plant, Anacyclus pyrethrum DC root


1 Department of Phytopharmacy and Phytomedicine, J. K. K. Munirajah Medical Research Foundation's, Annai J. K. K Sampoorani Ammal College of Pharmacy, B. Komarapalayam, Namakkal, Tamil Nadu, India
2 Department of Pharmaceutical Chemistry, Ultra College of Pharmacy, Madurai, Tamil Nadu, India

Date of Web Publication9-May-2014

Correspondence Address:
K G Lalitha
Department of Pharmaceutical Chemistry, Ultra College of Pharmacy, Madurai, Tamil Nadu
India
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Source of Support: None, Conflict of Interest: None


DOI: 10.4103/0253-7613.132204

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How to cite this article:
Kumar V K, Lalitha K G. In vitro study on α-amylase inhibitory activity of an Ayurvedic medicinal plant, Anacyclus pyrethrum DC root. Indian J Pharmacol 2014;46:350-1

How to cite this URL:
Kumar V K, Lalitha K G. In vitro study on α-amylase inhibitory activity of an Ayurvedic medicinal plant, Anacyclus pyrethrum DC root. Indian J Pharmacol [serial online] 2014 [cited 2020 Dec 1];46:350-1. Available from: https://www.ijp-online.com/text.asp?2014/46/3/350/132204


Sir,

Diabetes mellitus (DM) consists of a group of metabolic diseases characterized by inappropriate hyperglycemia resulting from defects in insulin secretion, insulin action or both. Perhaps food habits and genetic factors play a major role for diabetes. A study revealed that urbanization of rural India has doubled the rate of diabetes, in which type 2 DM is the primary threat to human health due to the increasing prevalence, chronic course and disabling complications. [1] The medicinal plants or natural products involve retarding the absorption of glucose by inhibiting the carbohydrate hydrolyzing enzymes, such as pancreatic amylase. The inhibition of this enzyme delays carbohydrate digestion and prolong overall carbohydrate digestion time, resulting in the reduction in glucose absorption rate and consequently dulling the postprandial plasma glucose rise. Several indigenous medicinal plants have high potential in inhibiting α-amylase enzyme activity. [2] Different parts of plant have been used predominantly in folk medicine worldwide for the treatment of various diseases such as paralysis, epilepsy, fever, pharyngitis, tonsillitis, and diabetes. [3] However, their antidiabetic activity has not been documented. This study was carried out to evaluate in vitro inhibitory effects of various extracts (petroleum ether, chloroform, ethyl acetate, acetone, ethanol, and water) of A. pyrethrum root on porcine pancreatic amylase activity.

The species for the proposed study, A. pyrethrum DC root was purchased from local market (M.A.S. Stores, Country drugs wholesale and retail, Erode, India) and authenticated by Prof. P. Jayaraman, Director, National Institute of Herbal Science, Chennai-45, (Ref. no: PARC/2011/896). The dried powdered plant root of A. pyrethrum DC was extracted using a soxhlet apparatus sequentially with petroleum ether, chloroform, ethyl acetate, acetone, ethanol, and water. Each extract was evaporated using rotary evaporator under reduced pressure. The preliminary phytochemical screening of extracts was carried out by chemical tests. [4] The dried extracts were dissolved in dimethyl sulfoxide to make different concentrations and subjected to α-amylase inhibitory assay [5] and the results were tabulated [Table 1].
Table 1:: á-amylase inhibitory effects of different extracts of Anacyclus
pyrethrum DC root


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In comparison of IC 50 value of α-amylase inhibitory effect of different extracts of A. pyrethrum DC root against porcine pancreatic amylase revealed that ethanol extract at higher concentration showed 88.26% (IC 50 -29.25 μg/ml) significant α-amylase inhibitory effect than the other extracts. Simultaneously, all extract showed appreciable α-amylase inhibitory activity except petroleum ether extract when compared with acarbose. It may be due to the presence of chemical constituents such as alkaloids, flavonoids, phytosteroids, and also glycosides. The plant-based α-amylase inhibitory offers a prospective therapeutic approach for the management of diabetes. [6] This study supports the Ayurvedic concept that A. pyrethrum DC root could be useful in management of diabetes. [7] Furthermore, the in vivo antidiabetic activity of these extracts needs to be assessed prior to clinical use.

 
  References Top

1.Mitra A. Effect of a composite of tulsi leaves, amla, bitter gourd, gurmur leaves, jamun fruit and seed in type 2 diabetic patients. J Clin Diagn Res 2007;6:511-20.  Back to cited text no. 1
    
2.Prashanth D, Padmaja R, Samiulla DS. Effect of certain plant extracts on alpha-amylase activity. Fitoterapia 2001;72:179-81.  Back to cited text no. 2
    
3.Anonymous. The Wealth of India: Raw Materials. Vol. I. New Delhi: CSIR Press; 1985. p. 248.  Back to cited text no. 3
    
4.Khandelwal KR. Practical Pharmacognosy Techniques and Experiments. Pune, India: Nirali Prakashan; 2000. p. 149-54.  Back to cited text no. 4
    
5.Tamil IG, Dineshkumar B, Nandhakumar M, Senthilkumar M, Mitra A. In vitro study on α-amylase inhibitory activity of an Indian medicinal plant, Phyllanthus amarus. Indian J Pharmacol 2010;42:280-2.  Back to cited text no. 5
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6.McCue P, Vattem D, Shetty K. Inhibitory effect of clonal oregano extracts against porcine pancreatic amylase in vitro. Asia Pac J Clin Nutr 2004;13:401-8.  Back to cited text no. 6
    
7.Anonymous. The Ayurvedic Pharmacopoeia of India-Part I. 1 st ed. New Delhi: The Controller of Publications; 1999. p. 1-2.  Back to cited text no. 7
    



 
 
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