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| RESEARCH ARTICLE |
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| Year : 2013 | Volume
: 45
| Issue : 4 | Page : 376-380 |
Protective effect of Clerodendrum colebrookianum Walp., on acute and chronic inflammation in rats
Lokesh Deb1, Amitabha Dey2, G Sakthivel3, Subrat Kumar Bhattamishra4, Amitsankar Dutta5
1 Pharmacology Laboratory, Medicinal Plants and Horticultural Resources Division, Institute of Bioresources and Sustainable Development, Department of Biotechnology, Government of India, Takyelpat, Imphal, Manipur, India
2 Pharmacology Laboratory, Medicinal Plants and Horticultural Resources Division, Institute of Bioresources and Sustainable Development, Department of Biotechnology, Government of India, Takyelpat, Imphal, Manipur; Department of Pharmacology, Roland Institute of Pharmaceutical Sciences, Berhampur, Orissa, India
3 Department of Nanotechnology, Noorul Islam Centre for Higher Education, Noorul Islam University, Kumaracoil, Thuckalay, Tamilnadu, India
4 Department of Pharmacology, Roland Institute of Pharmaceutical Sciences, Berhampur, Orissa, India
5 Department of Pharmacology, R. K. Pharmacy College, Azamgarh, Uttar Pradesh, India
Correspondence Address:
Lokesh Deb
Pharmacology Laboratory, Medicinal Plants and Horticultural Resources Division, Institute of Bioresources and Sustainable Development, Department of Biotechnology, Government of India, Takyelpat, Imphal, Manipur
India
 Source of Support: This work supported by Institute of Bioresources & Sustainable Development, Department of Biotechnology, Government of India, Takyelpat, Imphal, Manipur - 795001, India under core research project entitled: "Pharmacological evaluation of active constituents from bioresources used in traditional/folklore medicines of north eastern India and value addition for commercial exploitation" (Code- IBSD/PBD-4), Conflict of Interest: None  | Check |
DOI: 10.4103/0253-7613.115021
Aim: To evaluate antioxidant, anti-inflammatory potential of the aqueous extracts and its aqueous, n-butanol, ethyl-acetate, and chloroform fractions of Clerodendrum colebrookianum Walp. leaves.
Materials and Methods: In this present study, all the test samples were evaluated on in-vivo inflammatory model such as carrageenan and histamine-induced acute-inflammation and cotton pellet induced granuloma formation in albino male rats. Test samples were also employed in in-vitro assays like DPPH* free radical scavenging activity and COX inhibition assay.
Results: The test samples at the dose of 200mg/kg/p.o. were found to cause significant inhibition of carrageenan and histamine-induced inflammation and cotton pallet-induced granuloma formation on acute and chronic inflammation in rats. The test samples, except n-butanol fraction, exhibited inhibitory effect for both COX-1 and COX-2, in in-vitro assay but their percentage of inhibition values differs from each other. The test samples (aqueous extracts, aqueous, n-butanol, ethyl-acetate, and chloroform fractions) at 100 μg concentration exhibits 54.37%, 33.88%, 62.85%, 56.28%, and 57.48% DPPH* radical-scavenging effect respectively in in-vitro antioxidant study.
Conclusion: These observations established the anti-inflammatory effect of C. colebrookianum leaves in acute and chronic stages of inflammation by free radical scavenging and inhibition of COX-1 and COX-2.
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