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| RESEARCH ARTICLE
|Year : 2013 | Volume
| Issue : 4 | Page : 339-343
Effect of thiamine pyrophosphate on ischemia-reperfusion induced oxidative damage in rat kidney
Durdu Altuner1, Nihal Cetin1, Bahadir Suleyman1, Zeynep Aslan2, Ahmet Hacimuftuoglu2, Mine Gulaboglu3, Neslihan Isaoglu4, Ismail Demiryilmaz5, Halis Suleyman1
1 Department of Pharmacology, Faculty of Medicine, Recep Tayyip Erdogan University, Rize, Turkey
2 Department of Pharmacology, Faculty of Medicine, Ataturk University, Erzurum, Turkey
3 Department of Biochemistry, Faculty of Pharmacy, Ataturk University, Erzurum, Turkey
4 Department of Anesthesia, Nene Hatun Obstetrics and Gynecology Hospital, Erzurum, Turkey
5 Department of General Surgery, Ibni Sina Hospital, Kayseri, Turkey
Objectives: The biochemical effects of thiamine pyrophosphate on ischemia-reperfusion (IR) induced oxidative damage and DNA mutation in rat kidney tissue were investigated, and compared to thiamine.
Materials and Methods: Rats were divided into four groups: Renal ischemia-reperfusion (RIR); thiamine pyrophosphate + RIR (TPRIR); thiamine + RIR (TRIR); and sham group (SG).
Results: The results of biochemical experiments have shown that malondialdehyde (MDA) levels in rat kidney tissue after TRIR and TPRIR treatment were 7.2 ± 0.5 (P > 0.05) and 3.3 ± 0.3 (P < 0.0001) μmol/g protein, respectively. The MDA levels in the SG rat kidney tissue and in RIR group were 3.6 ± 0.2 (P < 0.0001) and 7.6 ± 0.6 μmol/g protein, respectively. Total glutathione (tGSH) levels in TRIR, TPRIR, SG, and RIR animal groups were 2.2 ± 0.3 (P > 0.05), 5.8 ± 0.4 (P < 0.0001), 6.2 ± 0.2 (P < 0.0001), and 1.7 ± 0.2 nmol/g protein, respectively. In the TRIR, TPRIR, SG, and RIR animal groups; 8-hydroxyguanine (8-OHGua)/Gua levels, which indicate mutagenic DNA, were 1.75 ± 0.12 (P > 0.05), 0.93 ± 0.1 (P < 0.0001), 0.85 ± 0.08 (P < 0.0001), and 1.93 ± 0.24 pmol/L, respectively.
Conclusions: It has been shown that thiamine pyrophosphate prevents increase in mutagenic DNA in IR induced oxidative damage, whereas thiamine does not have this effect.
Department of Pharmacology, Faculty of Medicine, Recep Tayyip Erdogan University, Rize
Source of Support: None, Conflict of Interest: None
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