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| RESEARCH ARTICLE |
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| Year : 2013 | Volume
: 45
| Issue : 4 | Page : 339-343 |
Effect of thiamine pyrophosphate on ischemia-reperfusion induced oxidative damage in rat kidney
Durdu Altuner1, Nihal Cetin1, Bahadir Suleyman1, Zeynep Aslan2, Ahmet Hacimuftuoglu2, Mine Gulaboglu3, Neslihan Isaoglu4, Ismail Demiryilmaz5, Halis Suleyman1
1 Department of Pharmacology, Faculty of Medicine, Recep Tayyip Erdogan University, Rize, Turkey
2 Department of Pharmacology, Faculty of Medicine, Ataturk University, Erzurum, Turkey
3 Department of Biochemistry, Faculty of Pharmacy, Ataturk University, Erzurum, Turkey
4 Department of Anesthesia, Nene Hatun Obstetrics and Gynecology Hospital, Erzurum, Turkey
5 Department of General Surgery, Ibni Sina Hospital, Kayseri, Turkey
Correspondence Address:
Halis Suleyman
Department of Pharmacology, Faculty of Medicine, Recep Tayyip Erdogan University, Rize
Turkey
 Source of Support: None, Conflict of Interest: None  | Check |
DOI: 10.4103/0253-7613.115005
Objectives: The biochemical effects of thiamine pyrophosphate on ischemia-reperfusion (IR) induced oxidative damage and DNA mutation in rat kidney tissue were investigated, and compared to thiamine.
Materials and Methods: Rats were divided into four groups: Renal ischemia-reperfusion (RIR); thiamine pyrophosphate + RIR (TPRIR); thiamine + RIR (TRIR); and sham group (SG).
Results: The results of biochemical experiments have shown that malondialdehyde (MDA) levels in rat kidney tissue after TRIR and TPRIR treatment were 7.2 ± 0.5 (P > 0.05) and 3.3 ± 0.3 (P < 0.0001) μmol/g protein, respectively. The MDA levels in the SG rat kidney tissue and in RIR group were 3.6 ± 0.2 (P < 0.0001) and 7.6 ± 0.6 μmol/g protein, respectively. Total glutathione (tGSH) levels in TRIR, TPRIR, SG, and RIR animal groups were 2.2 ± 0.3 (P > 0.05), 5.8 ± 0.4 (P < 0.0001), 6.2 ± 0.2 (P < 0.0001), and 1.7 ± 0.2 nmol/g protein, respectively. In the TRIR, TPRIR, SG, and RIR animal groups; 8-hydroxyguanine (8-OHGua)/Gua levels, which indicate mutagenic DNA, were 1.75 ± 0.12 (P > 0.05), 0.93 ± 0.1 (P < 0.0001), 0.85 ± 0.08 (P < 0.0001), and 1.93 ± 0.24 pmol/L, respectively.
Conclusions: It has been shown that thiamine pyrophosphate prevents increase in mutagenic DNA in IR induced oxidative damage, whereas thiamine does not have this effect.
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