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| RESEARCH ARTICLE
|Year : 2012 | Volume
| Issue : 6 | Page : 683-687
Protective effect of methanolic extract of Garcinia indica fruits in 6-OHDA rat model of Parkinson's disease
Bhaveshkumar V Antala1, Manishkumar S Patel1, Satish V Bhuva2, Shiv Gupta3, Samir Rabadiya4, Mangala Lahkar1
1 Department of Pharmacology and Toxicology, NIPER, Guwahati, Assam, India
2 Department of Biotechnology, NIPER, Ahmedabad, Gujarat, India
3 Department of Natural Product, NIPER, Ahmedabad, Gujarat, India
4 Institute of Pharmacy, Nirma University, Ahmedabad, Gujarat, India
Context: Several studies have reported that antioxidants play an important role in Parkinson's disease (PD). Garcinia indica extract is a natural antioxidant, the present study was undertaken to evaluate the neuroprotective effect of methanolic extract of Garcinia indica (GIM) against 6-hydroxydopamine (6-OHDA) neurotoxicity for striatal dopaminergic neurons in the rat.
Materials and Methods: Thirty adult Wistar rats were randomly divided into five groups namely control, 6-OHDA model, and GIM (100, 200, and 400 mg/kg body weight suspended in one ml of 0.1% carboxymethyl cellulose). The treatment was started three days before surgery and continued for next 14 days. The surgery was done on third day in all groups for administration of 6-OHDA into the right striatum and right substantia nigra, whereas control group injected with 6-OHDA vehicle. Various behavior and biochemical tests (Apomorphine-induced rotational behavior, Stepping test, Initiation time, Postural balance test, and Disengage time) were used to evaluate the neuroprotective effect of GIM. One-way analysis of variance (ANOVA) followed by Dunnett's test was used to compare inter-group differences. P<0.05 was considered as statistically significant.
Results: GIM had significant (P<0.05, P<0.01) preventive effect in biochemical tests, i.e., dopamine and its metabolites measurement and in various behavior tests, i.e., apomorphine-induced rotational behavior, stepping test, initiation time, postural balance test, and disengage time as compared to 6-OHDA-treated rats.
Conclusions: Our results demonstrated that GIM acted as an effective neuroprotective agent for striatal dopaminergic neurons in 6-OHDA lesioned rat model of PD.
Bhaveshkumar V Antala
Department of Pharmacology and Toxicology, NIPER, Guwahati, Assam
Source of Support: None, Conflict of Interest: None
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