IPSIndian Journal of Pharmacology
Home  IPS  Feedback Subscribe Top cited articles Login 
Users Online : 2141 
Small font sizeDefault font sizeIncrease font size
Navigate Here
  Search
 
  
Resource Links
   Similar in PUBMED
   Search Pubmed for
   Search in Google Scholar for
   Article in PDF (155 KB)
   Citation Manager
   Access Statistics
   Reader Comments
   Email Alert *
   Add to My List *
* Registration required (free)

 
In This Article
   References

 Article Access Statistics
    Viewed1641    
    Printed63    
    Emailed0    
    PDF Downloaded115    
    Comments [Add]    
    Cited by others 1    

Recommend this journal

 


 
 Table of Contents    
LETTER TO THE EDITOR
Year : 2012  |  Volume : 44  |  Issue : 3  |  Page : 425
 

Evaluation of the concomitant use of methotrexate and curcumin on Freund's complete adjuvant-induced arthritis and hematological indices in rats


1 Department of Pharmacology, Government Medical College, Bhavnagar, Gujarat, India
2 Department of Pharmacology, GMERS Medical College, Gotri, Vadodara, Gujarat, India

Date of Web Publication17-May-2012

Correspondence Address:
Tejas K Patel
Department of Pharmacology, GMERS Medical College, Gotri, Vadodara, Gujarat
India
Login to access the Email id

Source of Support: None, Conflict of Interest: None


DOI: 10.4103/0253-7613.96357

Rights and Permissions



How to cite this article:
Patel MK, Barvaliya MJ, Patel TK. Evaluation of the concomitant use of methotrexate and curcumin on Freund's complete adjuvant-induced arthritis and hematological indices in rats. Indian J Pharmacol 2012;44:425

How to cite this URL:
Patel MK, Barvaliya MJ, Patel TK. Evaluation of the concomitant use of methotrexate and curcumin on Freund's complete adjuvant-induced arthritis and hematological indices in rats. Indian J Pharmacol [serial online] 2012 [cited 2021 Mar 8];44:425. Available from: https://www.ijp-online.com/text.asp?2012/44/3/425/96357


Sir,

We have read the article "Evaluation of the concomitant use of methotrexate and curcumin on Freund's complete adjuvant-induced arthritis and hematological indices in rats" by Banji et al. [1] published in Indian Journal of Pharmacology with great interest. The methodology of the study has the following flaws which we would wish to state:



  1. Hypothesis of the study was a beneficial role of combination of curcumin and methotrexate (MTX) to hematological toxicity produced by MTX. It is stated that MTX cause the impairment in the hematological indices with the dose of 2 mg/kg in group IV. In group V, MTX (1 mg/kg) + curcumin (30 mg/kg) and in group VI, MTX (1 mg/kg) + curcumin (100 mg/kg) showed the improvement in hematological indices. It is difficult to understand the comparison of hematological toxicity produced by methotrexate 2 mg/ kg and the combination of methotrexate 1 mg/kg and curcumin. Hematological toxicity of MTX is dose dependent. Higher dose of MTX is more prone to cause hematological abnormalities like pancytopenia, while lower dose are more prone to cause megaloblastic anemia. [2] In the present study, the doses of MTX in groups V and VI are 50% less than those in group IV. We can assume that toxicity reduction in groups V and VI is not purely related to addition of curcumin. To nullify this bias, either author should have had one more group of MTX with dose of 1 mg/kg or dose of MTX should have been kept 2 mg/kg with curcumin.
  2. In this study, the authors have not mentioned the vehicle in which the curcumin was dissolved. The vehicle control group was of normal saline, so we can assume that curcumin was dissolved in normal saline. Curcumin is relatively insoluble in water, but dissolves in acetone, gum acacia, ethyl acetate, methanol, ethanol, n-butanol, isopropanol, hexane and dichloromethane. [3],[4] Is curcumin soluble in normal saline?
  3. Paw volume measured on various days in all the study groups is mentioned in [Table 1] by Banji et al. [1] We want to mention that paw volume on day 0 (baseline data) should also be presented in [Table 1]. The authors stated that the time course of arthritis after the administration of Freund's complete adjuvant showed swelling of hind paw from the ninth day. Paw volume on the ninth day is almost similar in all the groups and without baseline data it is difficult to show paw oedema on the ninth day. Paw volume of negative control (group 1) on the ninth day is comparable with other groups.


 
  References Top

1.Banji D, Pinnapureddy J, Banji OJ, Kumar AR, Reddy KN. Evaluation of the concomitant use of methotrexate and curcumin on Freund's complete adjuvant-induced arthritis and hematological indices in rats. Indian J Pharmacol 2011;43:546-50.  Back to cited text no. 1
[PUBMED]  Medknow Journal  
2.Tripathi KD. Essentials of Medical Pharmacology. 6 th ed. New Delhi: Jaypee Brothers Medical Publishers (P) Ltd; 2009. p. 823.  Back to cited text no. 2
    
3.CURCUMIN: Chemical and Technical Assessment (CTA). Available from: ftp://ftp.fao.org/es/esn/jecfa/cta/CTA_61_Curcumin.pdf. [Last cited on 2011 October 17].  Back to cited text no. 3
    
4.Venkatesan N. Curcumin attenuation of acute adriamycin myocardial toxicity in rats. Br J Pharmacol 1998;124:425-7.  Back to cited text no. 4
    



This article has been cited by
1 Epidemiology, risk factors and management of cardiovascular diseases in IBD
Siddharth Singh,Iftikhar J. Kullo,Darrell S. Pardi,Edward V. Loftus
Nature Reviews Gastroenterology & Hepatology. 2014;
[Pubmed] | [DOI]



 

Top
Print this article  Email this article
 

    

Site Map | Home | Contact Us | Feedback | Copyright and Disclaimer
Online since 20th July '04
Published by Wolters Kluwer - Medknow