IPSIndian Journal of Pharmacology
Home  IPS  Feedback Subscribe Top cited articles Login 
Users Online : 14792 
Small font sizeDefault font sizeIncrease font size
Navigate Here
  Search
 
  
Resource Links
 »  Similar in PUBMED
 »  Search Pubmed for
 »  Search in Google Scholar for
 »Related articles
 »  Article in PDF (385 KB)
 »  Citation Manager
 »  Access Statistics
 »  Reader Comments
 »  Email Alert *
 »  Add to My List *
* Registration required (free)

 
In This Article
 »  Abstract
 » Introduction
 »  Materials and Me...
 » Results
 » Discussion
 » Acknowledgement
 »  References
 »  Article Tables

 Article Access Statistics
    Viewed2335    
    Printed103    
    Emailed2    
    PDF Downloaded117    
    Comments [Add]    
    Cited by others 2    

Recommend this journal

 


 
 Table of Contents    
SHORT COMMUNICATION
Year : 2012  |  Volume : 44  |  Issue : 3  |  Page : 390-392
 

Effect of Terminalia catappa on lipid profile in transplanted fibrosarcoma in rats


1 Department of Pharmacology, Acharya and BM Reddy College of Pharmacy, Bangalore, Karnataka, India
2 Department of Parasitology, University of Malaysia, Kuala Lumpur, India
3 Chalapathi Institute of Pharmaceutical Sciences, Guntur, Andhra Pradesh, India

Date of Submission07-Jul-2011
Date of Decision10-Nov-2011
Date of Acceptance28-Feb-2012
Date of Web Publication17-May-2012

Correspondence Address:
Tigari Prakash
Department of Pharmacology, Acharya and BM Reddy College of Pharmacy, Bangalore, Karnataka
India
Login to access the Email id

Source of Support: None, Conflict of Interest: None


DOI: 10.4103/0253-7613.96345

Rights and Permissions

 » Abstract 

To evaluate the effect of an antitumor activity of Terminalia catappa on lipid lowering activity in transplanted fibrosarcoma in Wistar albino rats. Methylcholantherene-induced fibrosarcoma was transplanted in rats. After 30 th day when tumor became palpable, started the treatment of ethanolic extract of Terminalia catappa by orally (250 and 500 mg/kg) for a period of 20 days. The blood sample was collected on 21 st day, and the liver and the kidney were also removed for studying the lipid profile in serum and the tissues. The levels of total cholesterol, triglycerides and very low density lipoprotein (VLDL) were markedly elevated and high density lipoprotein (HDL) was markedly decreased in the serum of tumor bearing rats. Significant alterations were also observed in the lipid profile of liver and kidney. These changes were significantly reversed in Terminalia catappa (500 mg/kg) treated animals. The reversal of altered lipid levels to normal values in rats with experimentally induced tumor was showed antitumor activity by Terminalia catappa.


Keywords: Fibrosarcoma, lipid profile, methylcholantherene, Terminalia catappa


How to cite this article:
Naitik P, Prakash T, Kotresha D, Rao NR. Effect of Terminalia catappa on lipid profile in transplanted fibrosarcoma in rats. Indian J Pharmacol 2012;44:390-2

How to cite this URL:
Naitik P, Prakash T, Kotresha D, Rao NR. Effect of Terminalia catappa on lipid profile in transplanted fibrosarcoma in rats. Indian J Pharmacol [serial online] 2012 [cited 2021 Dec 1];44:390-2. Available from: https://www.ijp-online.com/text.asp?2012/44/3/390/96345



 » Introduction Top


A fibrosarcoma is a malignant (cancerous) tumor that originates in the connective fibrous tissue found at the ends of bones of the arms or legs, and then spreads to other surrounding soft tissues. Soft tissues include fat, muscles, tendons (bands of fiber that connect bones to muscle) nerves, joint tissue, blood vessels and other fibrous tissue. It most commonly affects either a lower legs or arms. [1]

Chemotherapy is a major treatment for cancer, and some of the plants like Catharanthus roseus, Podophyllum peltatum, Podophyllum emodii, Taxus brevifolia, Ochrosia elliptica and Campototheca acuminate have provide active principles used to control advanced stages of malignancies. [2] Most of the chemotherapeutic agents have been reported to exhibit several normal tissue toxicity, accompanied by undesirable side effects. Further, many of the potent antineoplastic drugs are highly expensive, mutagenic, carcinogenic and teratogenic. [3]

The leaves of the plant Terminalia catappa have been used as a folk medicine for treating dermatitis, hepatitis in India and in Philippines. Earlier studies showed that Terminalia catappa possess antioxidative, anti-inflammatory, and hepatoprotective activity. Leaves of Terminalia catappa possess anti - cancer, antioxidant as well as anti-clastogenic characteristics. [4],[5] Some flavonoids and hydrolyzable tannins have been identified in the Terminalia catap, [6] and earlier were reported, and it has shown that silibinin, a polyphenolic flavonoid isolated from milk thistle, inhibits the lung cancer metastasis. [7]

However, the literature survey offered no scientific claim on antitumor activity of Terminalia catappa against transplanted fibrosarcoma in rats. Therefore, the present study was planned to evaluate the antitumor activity of Terminalia catappa against effect on lipid profile in transplanted fibrosarcoma in rats.


 » Materials and Methods Top


Plant and Extraction

Leaves of plant Terminalia catappa were collected in the garden and authenticated by Dr. Jawahar Raveendran, Botanist, Bangalore, Karnataka, India. The leaves were shade-dried and made cores powdered. The powder was then packed into Soxhlet apparatus and subjected to hot continuous percolation using ethanol (95% v/v) as a solvent. The extract was concentrated under vacuum evaporator and stored in airtight container at 4°C in refrigerator.

Animal

Wistar albino rats of either sex (150 g - 200 g) were obtained from the National Institute of Mental Health and Neuro Science (NIMHANS), Bangalore. Rats were housed in polypropylene cages at controlled environment (temperature 25±2°C and 12 h dark/light cycle) and provided standard mice pellets and, water was allowed ad libitum. Experimental protocols were approved by our Institutional Animal Ethical Committee (Protocol No: 07/P'cology/09-10) and were used for the study.

Experimental Procedure

Induction of fibrosarcoma


Fibrosarcoma was induced in rats by the method of Nagarajan and Sankaran. [8] Methylcholantherene-induced fibrosarcomas maintained in rats were removed and minced in saline and prepared as a 10% suspension by homogenizing in a tissue homogenizer. 0.2 ml of this was injected into the inguinal region of the experimental animals. After 30 days, when the tumor developed into a palpable stage, the treatment was started. 2 doses of ethanolic extract of Terminalia catappa (250 and 500 mg/kg) were selected for treatment.

The animals were divided into 5 groups of 6 animals each. The first group consisted of normal rats while the second group had tumor bearing control animals, which received only the vehicle. The third and fourth groups (tumor bearing) were administered 250 and 500 mg/kg, orally of Terminalia catappa and fifth group received 5-Fluorouracil (20 mg/kg, orally), respectively, continuously for 20 days.

Estimation of lipids

On day 21, 24 h after the last dose blood was collected by cardiac puncture prior to sacrifice. The liver and the kidney were removed quickly, washed with ice-cold saline and preserved. Estimation of lipid parameters viz. total cholesterol, triglycerides, HDL, and VLDL in serum, liver, and kidney were carried out by using the biochemical kits (Span diagnostic Ltd, Mumbai, India).

Statistical Analysis

The statistical significance was assessed using one-way analysis of variance followed by Dennett's test. The values are expressed as mean ± SEM, and P<0.05 were considered to be statistically significant.


 » Results Top


The various parameters in the serum such as total cholesterol, triglycerides, and VLDL were found to be significantly elevated and HDL were significantly decreased in tumor-bearing rats [Table 1]. Terminalia catappa (500 mg/ kg) treatment restored these parameters to normal levels. In liver as well as in kidney, a significant reduction in the total cholesterol, triglycerides, HDL, and VLDL were observed in the tumor-bearing rats [Table 2]. Terminalia catappa (500 mg/ kg) restored all these values to normal levels while there was only a partial restoration of these values with the lower dose (250 mg/ kg). Terminalia catappa treatment was not statistically alters the HDL level in kidney. The alterations in the lipid parameters observed in the kidney were similar to those in the liver.
Table 1: Effect of ethanolic extract of Terminalia catappa on lipid levels in serum of fibrosarcoma bearing rats

Click here to view
Table 2: Effect of ethanolic extract of Terminalia catappa on lipid levels in liver and kidney of fibrosarcoma bearing rats

Click here to view



 » Discussion Top


The increased level of total cholesterol and triglycerides in fibrosarcoma-bearing control group may be explicated by reducing activities of fat splitting enzymes such as, lecithin: Cholesterol acetyltransferase (LCAT) and lipoprotein lipase. LCAT is responsible for an esterification of free cholesterol in plasma, and it indirectly controls the levels of free cholesterol in various cells and tissues. Lipoprotein lipase is the clearing factor for triglyceride in plasma and cleaves triglycerides into free fatty acids and glycerol. [9]

The increased levels of triglycerides observed in fibrosarcoma-bearing rats may be due to the increased synthesis of VLDL cholesterol. The decreased level of HDL cholesterol observed in untreated fibrosarcoma bearing rats may be explained by the parallel decreases in LDL cholesterol. Since, the reduction in HDL cholesterol is mostly compensated by an increment in LDL cholesterol, and vice versa. [10] The increased synthesis of very low density lipoprotein-cholesterol observed in fibrosarcoma-bearing control could have led to the increased triglyceride levels in fibrosarcoma-bearing animals. Finally, excessive lipid peroxides formed in fibrosarcoma condition may lead to hyper-lipidemia. [11]

Excessive rates of lipid peroxidation may be root of the hyperlipidemia, found in many cancer patients. [12] Terminalia catappa may have hypocholesterolemic effect, probably by an increase in the activity of cholesterol - 7-hydroxylase which increase the cholesterol.

In the tumor-bearing animals, increased lipolytic activity was reported, associated with hyper lipidemia. [13] The hyperlipidemic state was found to correlate with tumor burden and was reversible on tumor removal remission.

In the present study, a significant depletion of triglyceride, total cholesterol, HDL, VLDL from liver and kidney of the animals was observed, indicating an increased lipolytic activity in tumor-bearing animals. Correspondingly, the elevated levels of these lipids were observed in serum. The treatment of Terminalia catappa significantly attenuated these alterations of lipid levels in tissue as well as in serum. The depletion of fat stores associated with tumor growth indicates high energy requirement and hence an increased lipolytic activity. [14] The normalization of lipid level in liver and kidney tissues and serum upon Terminalia catappa treatment may be due to an enhanced lipogenesis or due to decrease in lipolysis, or both. These changes were significantly reversed in Terminalia catappa treated animals at oral dose of 500 mg/kg.

The results indicate that ethanolic extract of Terminalia catappa exhibited significant reversal of altered lipid levels near to normal values in rats with experimentally-induced fibrosarcoma.


 » Acknowledgement Top


The authors would like to express their gratitude to Premanath Reddy, Chairman Acharya Institute and Dr. Divakar Goli, Principal, Acharya and BM Reddy College of Pharmacy, Bangalore, India, for providing the necessary facilities and support to carry out the research work.

 
 » References Top

1.Bone and soft tissue Tumors: Malignant Tumors. Available from: http://www.dana-farber.org/pat/pediatric-care/pediatric-programs/bone-and-softtissue-tumors/fibrosarcoma.html. [Last Accessed on 2008 Sep 27].   Back to cited text no. 1
    
2.Jagetia GC, Rao SK. Evaluation of the antineoplastic activity of Guduchi (Tinospora cordifolia) in Ehrlich ascites carcinoma in mice. Biol Pharm Bull 2006;29:460-6.   Back to cited text no. 2
[PUBMED]  [FULLTEXT]  
3.Jagetia GC, Venkatesh P, Baliga MS. Aegle marmelos (L). Correa inhibits the proliferation of transplanted Ehrlich ascites carcinoma in mice. Biol Pharm Bull 2005;28:58-64.  Back to cited text no. 3
[PUBMED]  [FULLTEXT]  
4.Charng-Cherng C, Pei-Tzu K, Jeng-Leun M. Antioxident properties of solvent extract from Terminalia catappa leaves. Food Chem 2002;78:483-8.  Back to cited text no. 4
    
5.Ko TF, Weng YM, Lin SB, Chiou RY. Antimutagenicity of supercritical CO 2 extract of Terminalia catappa leaves and cytotoxicity of the extract to human hepatoma cells. J Agric Food Chem 2003;51:3564-7.  Back to cited text no. 5
[PUBMED]  [FULLTEXT]  
6.Tanaka T, Nonaka GI, Nishioka I. Tannins and related compounds. XLII. Isolation and characterization of four new hydrolysable tannins, terfIavins A and B, tergallagin and tercatain from the leaves of Termindia cutappo L. Chem Pharm Bull 1986;34:1039-49.  Back to cited text no. 6
    
7.Chu SC, Chiou HL, Chen PN, Yang SF, Hsieh YS. Silibinin inhibits the invasion of human lung cancer cells via decreased productions of urokinase-plasminogen activator and matrix metalloproteinase - 2. Mol Carcinog 2004;40:143-9.  Back to cited text no. 7
[PUBMED]  [FULLTEXT]  
8.Nagarajan B, Sankaran S. A rapid method for fibrosarcoma in rats and the effect of BCG administration. Indian J Cancer 1973;10:83-4.   Back to cited text no. 8
    
9.Clark RW, Crain CC. Characterization of alterations in plasma lipoprotein lipid and apolipoprotein profiles accompanying hepatoma induced hyperlipidemia in rats. Cancer Res 1998;46:1894-903.  Back to cited text no. 9
    
10.Bush TL, Friend. LP. Barrett-Connor, Cholesterol, lipoproteins and coronary heart disease in women. Clin Chem 1986;34:60-70.  Back to cited text no. 10
    
11.Vasavi H, Thangaraju M. Effect of tocopherol on lipid peroxidation and antioxidant system in fibrosarcoma bearing rats. Mol Cell Biochem 1994;131:125-9.  Back to cited text no. 11
    
12.Bast A, Haenen GR, Doelman CJ. Oxidants and antioxidants: State of the art. Am J Med 1991;91:S2-13.  Back to cited text no. 12
    
13.Devereux DF, Hollander DM. Effects of tumor bearing and removal on blood levels of lipids, lipolytic activity and glycerol on carcass weights in the rat. Surgery 1987;101:228-33.  Back to cited text no. 13
[PUBMED]    
14.Ekman L, Karlberg T, Edstrom S, Lindmark L, Schersten T, Lundholm K. Metabolic alterations in liver, skeletal muscle and fat tissue in response to different tumor burdens in growing sarcoma-bearing rats. J Surg Res 1982;33:23-31.  Back to cited text no. 14
    



 
 
    Tables

  [Table 1], [Table 2]

This article has been cited by
1 Terminalia catappa, an anticlastogenic agent against MMS induced genotoxicity in the human lymphocyte culture and in bone marrow cells of Albino mice
Mohammad Sultan Ahmad,Sheeba Ahmad,M. Arshad,K.B. Rai,Mohammad Afzal
Egyptian Journal of Medical Human Genetics. 2014;
[Pubmed] | [DOI]
2 Atividade antioxidante e teor de fenólicos de folhas da Terminalia catappa Linn em diferentes estágios de maturação | [Antioxidant activity and phenolic content of terminalia catappa linn leaves at different stages of maturation]
Ladeia Fogaça, D.N., Pinto Júnior, W.R., Rêgo Júnior, N.O., Sales Nunes, G.
Revista de Ciencias Farmaceuticas Basica e Aplicada. 2013; 34(2): 257-261
[Pubmed]



 

Top
Print this article  Email this article
 

    

Site Map | Home | Contact Us | Feedback | Copyright and Disclaimer
Online since 20th July '04
Published by Wolters Kluwer - Medknow