IPSIndian Journal of Pharmacology
Home  IPS  Feedback Subscribe Top cited articles Login 
Users Online : 14574 
Small font sizeDefault font sizeIncrease font size
Navigate Here
Resource Links
 »  Similar in PUBMED
 »  Search Pubmed for
 »  Search in Google Scholar for
 »Related articles
 »  Article in PDF (790 KB)
 »  Citation Manager
 »  Access Statistics
 »  Reader Comments
 »  Email Alert *
 »  Add to My List *
* Registration required (free)

In This Article
 »  Abstract
 » Introduction
 »  Materials and Me...
 » Results
 » Discussion
 » Conclusion
 » Acknowledgement
 »  References
 »  Article Figures
 »  Article Tables

 Article Access Statistics
    PDF Downloaded226    
    Comments [Add]    
    Cited by others 16    

Recommend this journal


 Table of Contents    
Year : 2012  |  Volume : 44  |  Issue : 1  |  Page : 78-81

Anti-tumor effects of Astragalus on hepatocellular carcinoma in vivo

1 Department of Medicine, Sichuan General Hospital of Armed Police Force, Chengdu, Sichuan, China
2 Department of Pharmacology, West China Center of Medical Sciences, Sichuan University, Chengdu, Sichuan, China

Date of Submission20-Jan-2011
Date of Decision03-Oct-2011
Date of Acceptance18-Oct-2011
Date of Web Publication14-Jan-2012

Correspondence Address:
Li-Hong Wan
Department of Pharmacology, West China Center of Medical Sciences, Sichuan University, Chengdu, Sichuan
Login to access the Email id

Source of Support: None, Conflict of Interest: None

DOI: 10.4103/0253-7613.91872

Rights and Permissions

 » Abstract 

Objective: The objective of the present study is to investigate the anti-proliferation activity of Astragalus on human hepatocellular carcinoma (HCC) cells and its mechanism. Materials and Methods: Hepatic cancer H22 bearing mice were used to study the anti-hepatocarcinoma activity of Astragalus in vivo. The growth curve and inhibitory rate of tumor growth were measured. Cell apoptosis of each group was measured by flow cytometry (FCM). Protein expression of Bax and Bcl-2 were analyzed by immunohistochemistry (IHC). The Statistical Package for Social Sciences version 13.0 (SPSS Inc, Chicago, IL) was used for standard statistical analysis including one-way ANOVA and Student's t-test. A value of P<0.05 was considered to be statistically significant.
Results: Astragalus significantly inhibited the growth of H22 carcinoma, with an inhibitory rate of 17.28-52.36%. FCM and immunohistochemical assay show that the cell apoptosis rate and protein expression of Bax and Bax/Bcl-2 ratio of H22 transplanted tumor in Astragalus treated group were significantly higher than the control group (P<0.05). The protein expression of Bcl-2 was significantly lower than control (P<0.05).
Conclusion: The results of the present study suggest that Astragalus has significant anti-tumor effect in vivo in inducing apoptosis of H22 tumor cells by promoting protein expression of Bax, decreasing protein expression of Bcl-2 gene, and markedly increasing the Bax/Bcl-2 ratio.

Keywords: Antitumor effects, apoptosis, Astragalus, H22

How to cite this article:
Li LK, Kuang WJ, Huang YF, Xie HH, Chen G, Zhou QC, Wang BR, Wan LH. Anti-tumor effects of Astragalus on hepatocellular carcinoma in vivo. Indian J Pharmacol 2012;44:78-81

How to cite this URL:
Li LK, Kuang WJ, Huang YF, Xie HH, Chen G, Zhou QC, Wang BR, Wan LH. Anti-tumor effects of Astragalus on hepatocellular carcinoma in vivo. Indian J Pharmacol [serial online] 2012 [cited 2023 Sep 22];44:78-81. Available from: https://www.ijp-online.com/text.asp?2012/44/1/78/91872

 » Introduction Top

Worldwide, hepatocellular carcinoma (HCC) is the fifth most common malignancy and the third most common cause of cancer-related death. Liver cancer is a major health problem and its incidence is increasing in China, although several trials have attempted to control its progression. [1] Furthermore, conventional treatments such as chemotherapy and radiotherapy can increase the risk of potential complications. [2] Hence, herbal medicines have attracted considerable interest as a novel anticarcinogenic agent because of their high efficacy, low toxicity and costs.

Radix astragali is a key herb in many traditional formulations in China, which was recorded more than 2000 years ago in Sheng Nong's Materia Medica. In recent years, Astragalus injection (AI), as an extract from Radix astragali, has been extensively used for clinical adjunctive therapy to ameliorate the side effects of antineoplastic drugs. [3] Several clinical studies showed that the active constituents of AI, including polysaccharides, astragalosides and total flavonoids [4] could ameliorate the side effects of chemotherapeutic drugs. Recently, there has been growing evidence that the Astragalus extract may be a potential anti-tumorigenic agent. For instance, Astragalus polysaccharides can prevent hepatocarcinogenesis in rats. [5] On the other hand, the total saponins of Radix astragali (AST) also exhibited anti-carcinogenic effects in HT-29 human colon cancer cells. [6] However, the precise anti-carcinogenic effect and mechanism of AI remains unclear.

In the present study, we aimed to examine the effect of AI on the growth of H22 transplanted tumor in mice, and to further elaborate its growth-inhibitory mechanism. Our key findings demonstrate that AI exerts inhibitory effects on HCC cells in vivo by inducing cell apoptosis. These results suggest that AI is an attractive candidate drug to prevent the tumor proliferation.

 » Materials and Methods Top


Astragalus injection was obtained from Diao Pharmaceutical Co., Ltd (Chengdu, China). Cyclophosphamide (CTX), (Jiangsu Hengrui Medicine Co., Ltd, batch number - 08122621) was dissolved in distilled water.


Kunming male mice, five-week old, weighing 18~22 g, were obtained from the Experimental Animal Center at Sichuan University. The mice were housed in plastic cages with hardwood chip bedding in an air-conditioned room at 23±2°C and 55±5% humidity, and with a 12 h light/dark cycle on basal diet (animal center). The animal handlings and experimental procedures were approved by the Animal Ethics Committee of Sichuan University (Permit Number: 2003-149).

In Vivo Anti-Tumor Activity

Five-week old male Kunming mice were inoculated with H22 cell suspension (1×10 7 /ml), 0.1 ml/10 g, through subcutaneous injection at right side axilla. Twenty-four hour after the tumor cell inoculation, the mice were randomized equally into 5 groups of 15 each. The minimum effective dose of Astragalus in treating hepatocellular carcinoma was 4 g/kg. According to the method of Prasad et al., [7] the positive control group mice received a single dose of cyclophosphamide (i.p., 600 mg/kg) on day 1. The negative control group mice were injected with an equal volume of normal saline (NS) while mice in other three groups were given AI (i.p., 12, 8, 4 g/kg) once every alternate day from day 1 (six times totally). From the fourth day, tumor volume (V) was measured on alternate days, and calculated according to the formula: V (cm 3 ) = ab 2 /2, where a is largest superficial diameter and b is smallest superficial diameter.

All mice were sacrificed 13 days after inoculation with H22 cells, and the transplanted tumors were excised and weighed. To evaluate the anticancer activity of Astragalus, tumor inhibitory rates were calculated with the following formula: tumor inhibitory rate (%) = 1-(tumor weight of treated group/ tumor weight of control group) ×100.

Flow Cytometry Analysis

Apoptotic rate was assessed by flow cytometry (FCM)after staining with propidium iodide (PI) and annexin VFITC (Sigma, St. Louis, MO), according to the method of Ji et al. Briefly, tumor tissues were immediately removed and the RBCs were lysed using an ammonium chloride lysis buffer and filtered through a double layer of stainless-steel mesh using a syringe plunger to obtain single cell suspension. [8] 1 × 10 6 cells per sample were incubated in 1× binding buffer containing Annexin V-FITC and PI. At least 5000 cells per sample were analyzed by flow cytometry. Data were analyzed with Flow Cytometry Software (Beckman Coulter) and the gating included all cells.

Measurement of Transplanted Tumors' BCL-2 and Bax Protein

Sections were cleared of paraffin, and endogenous peroxidases were blocked by incubation with 3% H 2 O 2 and washed. Sections of the tissues were then incubated with rabbit serum for 10 min at ambient temperature. Subsequently, the sections were incubated overnight with a goat polyclonal anti-Bax antibody (Boster, China, 1:50) and anti-Bcl-2 antibody (Boster, China, 1:50) at 4°C, followed by the addition of biotinylated rabbit anti-goat IgG secondary antibody (Jinshan, BJ, China). To verify the binding specificity for Bax and Bcl-2, some sections were also incubated with primary antibody or the secondary antibody. In these situations, no positive staining was found in the sections, indicating that the immunoreactions were positive in all the experiments. Immunohistochemistry staining was processed in accordance with the manufacturer's instructions and visualized by use of diaminobenzidine (DAB) staining. Counterstaining was carried out with Harris hematoxylin (Sigma). Ten sections were analyzed for each animal. Samples were observed in four randomly selected optical fields under microscopy (× 200) and the average optical density was analyzed with Image-pro plus (Media Cybernetics, USA) by an observer blind to the treatment groups.

Statistical Analysis

The data were expressed as mean±standard deviation (SD). The Statistical Package for Social Sciences version 13.0 (SPSS Inc, Chicago, IL) was used for standard statistical analysis including one-way ANOVA and Student's t-test. A value of P <0.05 was considered as statistically significant.

 » Results Top

Growth of Mouse Transplantable Hepatoma

To determine whether Astragalus inhibits tumor growth in vivo, H22 cells were injected into Kunming mice. Growth curve of hepatocarcinoma H22 showed that Astragalus inhibited tumor growth significantly in a dose- and time-dependent manner from 4 to 12 g/kg [Figure 1]. Treated with Astragalus, the tumor inhibition rates ranged from 17.28 to 52.36% [Table 1].
Figure 1: Effects of different dosages of Astragalus injection and cyclophosphamide (CTX) on tumor growth in H22 bearing mice. *P < 0.05 vs control

Click here to view
Table 1: Inhibitory effect of Astragalus injection on H22 bearing mice

Click here to view

Flow cytometry analysis

After Astragalus treatment for 10 days, a significantly high rate of apoptosis was found, compared with saline controls in hepatocarcinoma H22 bearing mice [Figure 2].
Figure 2: Apoptosis rates of H22 transplanted tumor cells, values are shown as mean ± SD (n=5). *P < 0.05 vs control. CTX=cyclophosphamide (60 mg/kg); AIH=Astragalus injection (12g/kg); AIM=Astragalus injection (8 g/kg); AIL=Astragalus injection (4 g/kg).

Click here to view

Effects of AI onBcl-2 and Bax protein level

To test the effect of AI on the regulation of apoptosis gene, immunohistochemistry staining was performed for Bcl-2 and Bax protein. [Figure 3] shows that the protein levels of Bcl-2 and Bax protein changed in Astragalus treated H22 bearing mice.AI treatment decreased the expression of anti-apoptotic protein Bcl-2 compared with the control (P< 0.05). The expression of the pro-apoptotic protein Bax was increased significantly by AI treatment in a dose-dependent manner as compared with control (P < 0.05). A marked enhancement of the Bax/Bcl-2 ratio was observed after AI treatment [Figure 3]. There was no significant difference among the different doses of AI.
Figure 3: Expression of Bax and Bcl-2 proteins in transplanted tumor cells of H22 bearing mice (a) Representative immunostaining for Bax proteins in H22 bearing mice (×200. (b) Representative immunostaining for Bcl-2 proteins in H22 bearing mice (×200. (c) Semi-quantitative analysis of the optical density of Bax. (d) Semi-quantitative analysis of the optical density of of Bcl-2. (e) The calculated Bax/Bcl-2 ratio. Values are shown as mean±SD (n=10). *P < 0.05 vs control.CTX=cyclophosphamide (60 mg/kg); AIH= Astragalus injection (12 g/kg); AIM=Astragalus injection (8 g/kg); AIL= Astragalus injection (4 g/kg).

Click here to view

 » Discussion Top

Apoptosis, the program of cellular suicide, is critical in tumorigenesis and tumor progression. [9] Thus, targeting apoptosis is considered as an effective therapeutic strategy for treatment of cancer. [10],[11] Astragalus, a traditional Chinese medicine, has so far been used as a modulating agent for all kinds of immunological diseases and in combination with cytotoxic drugs to ameliorate their side effects. [3] Recently, it has been demonstrated that the anti-tumor mechanism of Astragalus may be an induction of the apoptosis of cancer cell. [12],[13],[14] However, there has been little scientific advancement with regard to the effect of Astragalus on cancer. Among the effective components of Astragalus mongholicus, total Astragalus saponins (AST) possess anti-tumor properties on human colon cancer cells and human hepatocellular HepG2 cell line by inducing apoptosis in vitro. [6],[13],[15] Astragalus injection is the water extract of Astragalus mongholicus. Astragalus saponins are the main component of Astragalus injection. Our study demonstrated that both the weight and volume of tumor were inhibited by Astragalus in a dose- and time-dependent manner from 4 to 12 g/kg. Moreover, Astragalus injection could induce apoptosis of H22 transplanted tumors cells. The apoptosis rate also exhibited a dose- and time-dependence.

To obtain further information regarding apoptotic modulation, we have examined the immunoreactivity of Bax and Bcl-2 proteins in H22 transplanted tumors. Bcl-2 and Bax function as tumor anti-apoptotic and pro-apoptotic factors and are the two main members of the Bcl-2 protein family, which might interact with each other to form homodimers and heterodimers in regulating apoptosis. [16],[17] Therefore, alteration in the levels of Bax and Bcl-2 protein influences apoptosis [18] and the ratio between them is the key to determine the cell survival or death. [19],[20] In our study, we showed the Astragalus injection induced apoptosis in H22 transplanted tumors and it was accompanied by up-regulation of Bax and the down-regulation of Bcl-2. The ratio of Bax/Bcl-2 also increased after Astragalus injection. These results indicate that imbalance of Bcl-2 family protein expression plays a critical role in this anti-tumor treatment. Astragalus injection induced apoptosis in H22 transplanted tumors by modulating Bcl-2 family proteins.

 » Conclusion Top

In the present study, the antitumor effect and the preliminary growth-inhibitory mechanism of Astragalus injection on H22 bearing mice were investigated. From the results, it is concluded that Astragalus injection can inhibit tumor growth by inducing apoptosis via up-regulating Bax expression and the ratio of Bax/Bcl-2 and down-regulating Bcl-2 expression. It is suggested that Astragalus injection can be an attractive candidate for cancer therapy. Further investigation of Astragalus injection as an anti-tumor compound is warranted.

 » Acknowledgement Top

The authors gratefully acknowledge the encouragement of Pro. Liming Zhou, Director, Department of Pharmacology, West China Centre of Medical Sciences, Sichuan University, Chengdu, China.

 » References Top

1.Shariff MI, Cox IJ, Gomaa AI, Khan SA, Gedroyc W, Taylor-Robinson SD. Hepatocellular carcinoma: Current trends in worldwide epidemiology, risk factors, diagnosis and therapeutics. Expert Rev Gastroenterol Hepatol 2009;3:353-67.  Back to cited text no. 1
2.Lin DY, Lin SM, Liaw YF. Non-surgical treatment of hepatocellular carcinoma. J Gastroenterol Hepatol 1997;12:S319-28.  Back to cited text no. 2
3.Zee-Cheng RK. Shi-quan-da-bu-tang (ten significant tonic decoction), SQT. A potent Chinese biological response modifier in cancer immunotherapy, potentiation and detoxification of anticancer drugs. Methods Find Exp Clin Pharmacol 1992;14:725-36.  Back to cited text no. 3
4.Zhang RP, Zhang XP, Ruan YF, Ye SY, Zhao HC, Cheng QH, et al. Protective effect of Radix Astragali injection on immune organs of rats with obstructive jaundice and its mechanism. World J Gastroenterol 2009;15:2862-9.  Back to cited text no. 4
5.Cui R, He J, Wang B, Zhang F, Chen G, Yin S, et al. Suppressive effect of Astragalus membranaceus Bunge on chemical hepatocarcinogenesis in rats. Cancer Chemother Pharmacol 2003;51:75-80.   Back to cited text no. 5
6.Tin MM, Cho CH, Chan K, James AE, Ko JK. Astragalus saponins induce growth inhibition and apoptosis in human colon cancer cells and tumour xenograft. Carcinogenesis 2007;28:1347-55.  Back to cited text no. 6
7.Prasad SB, Rosangkima G, Nicol BM. Cyclophosphamide and ascorbic acid-mediated ultrastructural and biochemical changes in Dalton's lymphoma cells in vivo. Eur J Pharmacol 2010;645:47-54.  Back to cited text no. 7
8.Ji DB, Ye J, Jiang YM, Qian BW. Anti-tumor effect of Liqi, a traditional Chinese medicine prescription, in tumor bearing mice. BMC Complement Altern Med 2009;9:20.  Back to cited text no. 8
9.Green DR, Evan GI.A matter of life and death. Cancer Cell 2002;1:19-30.  Back to cited text no. 9
10.Macha MA, Matta A, Sriram U, Thakkar A, Shukla NK, Datta Gupta S, et al. Clinical significance of TC21 overexpression in oral cancer. J Oral Pathol Med 2010;39:477-85.  Back to cited text no. 10
11.Kuhar M, Sen S, Singh N. Role of mitochondria in quercetin-enhanced chemotherapeutic response in human non-small cell lung carcinoma H-520 cells. Anticancer Res 2006;26:1297-303.  Back to cited text no. 11
12.Song Y, Yang J, Bai WL, Ji WY. Anti-tumour and immunoregulatory effects of Astragalus on nasopharyngeal carcinoma In Vivo and In Vitro. Phytother Res 2011;25:909-15.  Back to cited text no. 12
13.Auyeung KK, Mok NL, Wong CM, Cho CH, Ko JK. Astragalus saponins modulate mTOR and ERK signaling to promote apoptosis through the extrinsic pathway in HT-29 colon cancer cells. Int J Mol Med 2010;26:341-9.  Back to cited text no. 13
14.Hu YW, Liu CY, Du CM, Zhang J, Wu WQ, Gu ZL. Induction of apoptosis in human hepatocarcinoma SMMC-7721 cells in vitro by flavonoids from Astragalus complanatus. J Ethnopharmacol 2009;123:293-301.  Back to cited text no. 14
15.Auyeung KK, Law PC, Ko JK. Astragalus saponins induce apoptosis via an ERK-independent NF-kappaB signaling pathway in the human hepatocellular HepG2 cell line. Int J Mol Med 2009;3:189-96.  Back to cited text no. 15
16.Kim KW, Moretti L, Mitchell LR, Jung DK, Lu B. Combined Bcl-2/mammalian target of rapamycin inhibition leads to enhanced radiosensitization via induction of apoptosis and autophagy in non-small cell lung tumor xenograft model. Clin Cancer Res 2009;15:6096-105.  Back to cited text no. 16
17.Snyder CM, Shroff EH, Liu J, Chandel NS. Nitric oxide induces cell death by regulating anti-apoptotic BCL-2 family members. PLoS One 2009;4:e7059.  Back to cited text no. 17
18.Burlacu A. Regulation of apoptosis by Bcl-2 family proteins. J Cell Mol Med 2007;7:249-57.  Back to cited text no. 18
19.Reed JC. Dysregulation of apoptosis in cancer. J Clin Oncol 1999;17:2941-53.  Back to cited text no. 19
20.Korsmeyer SJ. BCL-2 gene family and the regulation of programmed cell death. Cancer Res 1999;59:1693s-700.  Back to cited text no. 20


  [Figure 1], [Figure 2], [Figure 3]

  [Table 1]

This article has been cited by
1 A traditional prescription comprising Astragali Radix and Schisandra chinensis Fructus induces apoptosis and protective autophagy in hepatocellular carcinoma cells
Pei-Li Zhu, Jun-Kui Li, Xiao-Li Jiang, Shi-Qing Zhang, Zhu Zhang, Ying Wang, Zhang Zhang, Wen-Qing Chen, Ken-Kin-Lam Yung
Journal of Ethnopharmacology. 2023; 312: 116548
[Pubmed] | [DOI]
2 A mini-review of traditional Chinese medicines on liver diseases
Siu Kan Law, Dawn Ching Tung Au, Albert Wing Nang Leung, Chuanshan Xu
Pharmacological Research - Modern Chinese Medicine. 2023; 7: 100243
[Pubmed] | [DOI]
3 Immunohistochemical Evaluation of the Euphorbia Inarticulata Extract on Liver and Kidney Tissues in Hepatocellular Carcinoma Rats
Ghaliahussine Almalki, Samar Rabah, Nadia Mohamed Said Arafa, Safia Mohammed Bahshwan
Pharmacophore. 2022; 13(2): 33
[Pubmed] | [DOI]
4 Development and Validation of a New Analytical HPLC-PDA Method for Simultaneous Determination of Cucurbitacins B and D from the Roots of Trichosanthes kirilowii
Hyoung Ja Kim, Jae Yeol Lee, Chunhoo Cheon, Seong-Gyu Ko, Shayessteh Dadfarnia
Journal of Chemistry. 2022; 2022: 1
[Pubmed] | [DOI]
5 Pharmacokinetic herb-drug interactions between Aidi injection and doxorubicin in rats with diethylnitrosamine-induced hepatocellular carcinoma
Yuan Lu, Jie Pan, Xiaoqing Zhu, Shuai Zhang, Chunhua Liu, Jia Sun, Yueting Li, Siying Chen, Jing Huang, Chuang Cao, Yonglin Wang, Yongjun Li, Ting Liu
BMC Pharmacology and Toxicology. 2021; 22(1)
[Pubmed] | [DOI]
6 SH003 overcomes drug resistance and immune checkpoints by inhibiting JAK-STAT3 signaling in MCF7/ADR cells
Jin Mo Ku, Se Hyang Hong, Hyo In Kim, Min Jeong Kim, Su-Jeong Ku, Kwang-Rok Bae, Hye Sook Seo, Yong Cheol Shin, Seong-Gyu Ko
Phytomedicine Plus. 2021; 1(4): 100111
[Pubmed] | [DOI]
7 Astragalus-Containing Chinese Herbal Medicine Combined With Chemotherapy for Cervical Cancer: A Systematic Review and Meta-Analysis
Lei Shen, Si Ra Gwak, Zhen Yang Cui, Jong Cheon Joo, Soo Jung Park
Frontiers in Pharmacology. 2021; 12
[Pubmed] | [DOI]
8 Combining the Anticancer and Immunomodulatory Effects of Astragalus and Shiitake as an Integrated Therapeutic Approach
Biju Balakrishnan, Qi Liang, Kevin Fenix, Bunu Tamang, Ehud Hauben, Linlin Ma, Wei Zhang
Nutrients. 2021; 13(8): 2564
[Pubmed] | [DOI]
9 Efficacy of Herbal Medicine as an Adjunctive Therapy of Chemotherapy for Cervical Cancer: A Systematic Review and Meta-analysis
Seung Yun Oh, Mi Suk Kim, Jong Cheon Joo, Yung Sun Song
Journal of Physiology & Pathology in Korean Medicine. 2020; 34(5): 255
[Pubmed] | [DOI]
10 Targeting Tumor Immunosuppressive Microenvironment for the Prevention of Hepatic Cancer: Applications of Traditional Chinese Medicines in Targeted Delivery
Le-Yi Zhang, Jun-Gang Zhang, Xue Yang, Mao-Hua Cai, Cheng-Wu Zhang, Zhi-Ming Hu
Current Topics in Medicinal Chemistry. 2020; 20(30): 2789
[Pubmed] | [DOI]
11 SH003 reverses drug resistance by blocking signal transducer and activator of transcription 3 (STAT3) signaling in breast cancer cells
Hye-Sook Seo, Jin Mo Ku, Hee-Jae Lee, Jong-Kyu Woo, Chunhoo Cheon, Mia Kim, Bo-Hyoung Jang, Yong Cheol Shin, Seong-Gyu Ko
Bioscience Reports. 2017; 37(6)
[Pubmed] | [DOI]
12 Enhanced Antitumor Efficacy with Combined Administration of Astragalus and Pterostilbene for Melanoma
Xin-Yan Huang,Song-Zhao Zhang,Wen-Xi Wang
Asian Pacific Journal of Cancer Prevention. 2014; 15(3): 1163
[Pubmed] | [DOI]
13 Herbal Extract SH003 Suppresses Tumor Growth and Metastasis of MDA-MB-231 Breast Cancer Cells by Inhibiting STAT3-IL-6 Signaling
Youn Kyung Choi,Sung-Gook Cho,Sang-Mi Woo,Yee Jin Yun,Sunju Park,Yong Cheol Shin,Seong-Gyu Ko
Mediators of Inflammation. 2014; 2014: 1
[Pubmed] | [DOI]
14 The Standardized BHH10 Extract, a Combination ofAstragalus membranaceus,Cinnamomum cassia, andPhellodendron amurense, Reverses Bone Mass and Metabolism in a Rat Model of Postmenopausal Osteoporosis
Jeong-Eun Huh,Soo-Jeong Kim,Jung-Won Kang,Dong-Woo Nam,Do-Young Choi,Dong-Suk Park,Jae-Dong Lee
Phytotherapy Research. 2014; : n/a
[Pubmed] | [DOI]
15 CD4+CD25+Foxp3+ T cells contribute to the antiasthmatic effects of Astragalus membranaceus extract in a rat model of asthma
Hualiang Jin,Qingli Luo,Yijie Zheng,Muhammat Nurahmat,JinFeng Wu,Bei Li,YuBao Lv,Genfa Wang,Xiaohong Duan,JingCheng Dong
International Immunopharmacology. 2013; 15(1): 42
[Pubmed] | [DOI]
16 A New Herbal Formula, KSG-002, Suppresses Breast Cancer Growth and Metastasis by Targeting NF-?B-Dependent TNFa Production in Macrophages
Sang-Mi Woo,Youn Kyung Choi,Sung-Gook Cho,Sunju Park,Seong-Gyu Ko
Evidence-Based Complementary and Alternative Medicine. 2013; 2013: 1
[Pubmed] | [DOI]


Print this article  Email this article


Site Map | Home | Contact Us | Feedback | Copyright and Disclaimer | Privacy Notice
Online since 20th July '04
Published by Wolters Kluwer - Medknow