| RESEARCH ARTICLE
|Year : 2011 | Volume
| Issue : 6 | Page : 683-688
Kaurenic acid: Evaluation of the in vivo and in vitro antitumor activity on murine melanoma
Miriam C Sosa-Sequera1, Miguel Chiurillo2, Jaime Moscoso1, Josefina Dolinar1, Omar Suarez1, Natalia Neira1, Hernan Mendoza2, Marķa Rivero-Paris2
1 Research Unit in Experimental Pharmacology, Department of Functional Sciences, School of Medicine, Universidad Centroccidental Lisandro Alvarado (UCLA), Barquisimeto, Venezuela
2 Molecular Genetic Laboratory Dr. Jorge Yunis-Turbay, Department of Functional Sciences, School of Medicine, Universidad Centroccidental Lisandro Alvarado (UCLA), Barquisimeto, Venezuela
Objective : The in vivo and in vitro antitumor activity of kaurenic acid [kaur 16-en-19 oic acid] (KA) in melanoma was evaluated in a murine model in comparison with taxol (Tx).
Materials and Methods : B16F1 melanoma was developed in C57BL/6 mice and cell cultures. Survival test, tumor growth, dissected-tumor measurements, histology, cytoxicity assay on cultured cells, and changes of apoptotic gene expression at mRNA level were analyzed.
Results : KA showed antitumor effect in vivo and in vitro and compared with Tx, its antimelanoma activity was greater (P < 0.001). These results were confirmed by morphological analysis (P < 0.001). In melanoma cell cultures, KA IC 50 was 0.79 μM vs. 18.94 μM for Tx (P < 0.001). RT-PCR analysis demonstrated that Bcl-xL mRNA expression was altered in B16F1 mouse melanoma cells obtained from mice treated with either KA or Tx.
Conclusion : The data suggest that KA is active in animal melanoma models, both in vitro and in vivo, being its cytotoxic effects stronger than those exhibited by Tx. Further trials should be conducted to elucidate its mechanism of action in melanoma with respect to necrosis or apoptotic processes. Our results support other evidences indicating that KA is a potential chemotherapeutic agent against cancer that has to be widely explored.
Miriam C Sosa-Sequera
Research Unit in Experimental Pharmacology, Department of Functional Sciences, School of Medicine, Universidad Centroccidental Lisandro Alvarado (UCLA), Barquisimeto
Source of Support: Research Council (CDCHT) of the
Universidad Centroccidental Lisandro Alvarado (015-ME-2004 and
003-ME-2006), Conflict of Interest: None
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