| RESEARCH ARTICLE
|Year : 2011 | Volume
| Issue : 6 | Page : 676-682
Neuroprotective activity of Wedelia calendulacea on cerebral ischemia/reperfusion induced oxidative stress in rats
Tigari Prakash1, Dupadahalli Kotresha2, Rama Rao Nedendla3
1 Department of Pharmacology, Acharya and B.M. Reddy College of Pharmacy, Bangalore - 560 090, Karmataka, India
2 Department of Biochemistry, Indian Institute of Science, Bangalore - 560 012, Karnataka, India
3 Department of Pharmaceutical Chemistry, Chalapathi Institute of Pharmaceutical Science, Guntur - 522 034, Andhra Pradesh, India
Objective : This study was undertaken to evaluate the neuroprotective activity of Wedelia calendulacea against cerebral ischemia/reperfusion induced oxidative stress in the rats.
Materials and Methods : The global cerebral ischemia was induced in male albino Wistar rats by occluding the bilateral carotid arteries for 30 min followed by 1 h and 4 h reperfusion. At various times of reperfusion, the histopathological changes and the levels of malondialdehyde (MDA), glutathione peroxidase (GPx), glutathione reductase (GR), glutathione-s-transferase (GST), and hydrogen peroxide (H 2 O 2 ) activity and brain water content were measured.
Results : The ischemic changes were preceded by increase in concentration of MDA, hydrogen peroxide and followed by decreased GPx, GR, and GST activity. Treatment with W. calendulacea significantly attenuated ischemia-induced oxidative stress. W. calendulacea administration markedly reversed and restored to near normal level in the groups pre-treated with methanolic extract (250 and 500 mg/kg, given orally in single and double dose/day for 10 days) in dose-dependent way. Similarly, W. calendulacea reversed the brain water content in the ischemia reperfusion animals. The neurodegenaration also conformed by the histopathological changes in the cerebral-ischemic animals.
Conclusion : The findings from the present investigation reveal that W. calendulacea protects neurons from global cerebral-ischemic injury in rat by attenuating oxidative stress.
Department of Pharmacology, Acharya and B.M. Reddy College of Pharmacy, Bangalore - 560 090, Karmataka
Source of Support: None, Conflict of Interest: None
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