| RESEARCH ARTICLE
|Year : 2009 | Volume
| Issue : 1 | Page : 32-35
Leishmanicidal activity of saponins isolated from the leaves of Eclipta prostrata and Gymnema sylvestre
Venkatesan Gopiesh Khanna1, Krishnan Kannabiran1, Giulia Getti2
1 School of Biotechnology, Chemical and Biomedical Engineering, VIT University, Vellore, Tamil Nadu, India
2 School of Health and Bioscience, University of East London, Stratford Campus, Ramford Road, London, United Kingdom
Objective: To evaluate the leishmanicidal activity of saponin, dasyscyphin C of Eclipta prostrata and sapogenin, gymnemagenol from Gymnema sylvestre leaves under in vitro conditions.
Materials and Methods: Dasyscyphin C/Gymnemagenol were dissolved in phosphate buffered saline (PBS) and diluted with liquid medium to obtain concentrations ranging from 1000 to 15 µg /ml. The leishmanicidal activity against leishmanial parasites, Leishmania major, Leishmania aethiopica and Leishmania tropica promastigotes was studied by the MTS assay.
Result: The Dasyscyphin C isolated from E. prostrata showed good leishmanicidal activity at 1000µg/ml concentration, with the IC 50 value of 450µg/ml against L. major promastigote and the percentage of parasitic death was 73; whereas, gymnemagenol of G. sylvestre showed only 52% parasitic death at 1000 µg/ml concentration. The other Leishmania species, L. aethiopica and L. tropica promastigotes, were less sensitive to the saponins of E. prostrata and G. sylvestre.
Conclusion: From this study, it can be concluded that the dasyscyphin C of E. prostrata has significant leishmanicidal activity against L. major promastigote.
School of Biotechnology, Chemical and Biomedical Engineering, VIT University, Vellore, Tamil Nadu
Source of Support: None, Conflict of Interest: None
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