IPSIndian Journal of Pharmacology
Home  IPS  Feedback Subscribe Top cited articles Login 
Users Online : 9810 
Small font sizeDefault font sizeIncrease font size
Navigate Here
 » Next article
 » Previous article 
 » Table of Contents
Resource Links
 »  Similar in PUBMED
 »  Search Pubmed for
 »  Search in Google Scholar for
 »Related articles
 »  Article in PDF (134 KB)
 »  Citation Manager
 »  Access Statistics
 »  Reader Comments
 »  Email Alert *
 »  Add to My List *
* Registration required (free)

In This Article
 »  Abstract
 »  Introduction
 »  Materials and Me...
 »  Results
 »  Discussion
 »  References
 »  Article Figures
 »  Article Tables

 Article Access Statistics
    PDF Downloaded678    
    Comments [Add]    
    Cited by others 24    

Recommend this journal


Year : 2008  |  Volume : 40  |  Issue : 3  |  Page : 121-125

Polyamines: Potential anti-inflammatory agents and their possible mechanism of action

Department of Pharmacology and Toxicology, Prin. K. M. Kundnani College of Pharmacy, Jote Joy Building, Rambhau Salgaonkar Marg, Cuffe Parade, Mumbai - 400 005, India

Date of Submission07-Sep-2007
Date of Decision09-Jun-2008
Date of Acceptance21-Jun-2008

Correspondence Address:
Suresh Ramnath Naik
Department of Pharmacology and Toxicology, Prin. K. M. Kundnani College of Pharmacy, Jote Joy Building, Rambhau Salgaonkar Marg, Cuffe Parade, Mumbai - 400 005
Login to access the Email id

Source of Support: None, Conflict of Interest: None

DOI: 10.4103/0253-7613.42305

Rights and Permissions

 » Abstract 

Objective: To evaluate the anti-inflammatory activity of exogenously administered polyamines on experimentally induced acute and chronic inflammation in wistar rats and to elucidate their possible mechanism of action.
Materials and Methods: The in vivo anti-inflammatory activity of polyamines was studied using acute (carrageenin paw edema), sub-acute (cotton pellet granuloma) and chronic (Freund's adjuvant induced arthritis) models of inflammation. The biochemical parameters like liver lipid peroxides, SGOT and SGPT were also measured.
Results: Polyamines exhibited significant anti-inflammatory activity in acute, sub-acute and chronic models of inflammation. Polyamines treatment inhibited the increase in lipid peroxides in liver and the serum concentration of marker enzymes (glutamate oxaloacetate transferase and glutamate pyruvate transferase) during inflammation.
Conclusion: Polyamines possess anti-inflammatory activity in acute and chronic inflammation which can be attributed to their anti-oxidant and /or lysosomal stabilization properties.

Keywords: Arthritis, carrageenin paw edema, cotton pellet granuloma, lipid peroxides and lysosomal enzymes, polyamines

How to cite this article:
Lagishetty CV, Naik SR. Polyamines: Potential anti-inflammatory agents and their possible mechanism of action. Indian J Pharmacol 2008;40:121-5

How to cite this URL:
Lagishetty CV, Naik SR. Polyamines: Potential anti-inflammatory agents and their possible mechanism of action. Indian J Pharmacol [serial online] 2008 [cited 2023 Oct 4];40:121-5. Available from: https://www.ijp-online.com/text.asp?2008/40/3/121/42305

 » Introduction Top

The polyamines putrescine, spermidine and spermine are aliphatic polycations derived from ornithine and play vital physiological roles. [1] Polyamines being cationic in nature bind to the negatively charged nucleic acids of the cell membrane surface and are thus involved in stabilization of the membrane structures. [2] Polyamines are involved in cellular processes such as DNA and protein synthesis. They participate in cell proliferation and differentiation. [3] They also act as scavengers of reactive oxygen species and thereby protect DNA, proteins and lipids from oxidative damage. [4]

Polyamines have been postulated to have anti-inflammatory and anti-oxidant properties. [1] It has been suggested that they exert at least two different anti-inflammatory mechanisms, the first one is mediated by the synthesis of an anti-inflammatory protein (vasoregulin) [5] and the second one is their direct action on leucocytes. [6] Most of the reported studies however have been conducted on several cell-lines in vitro and those in vivo have been of a preliminary nature. Also, they have not been investigated in chronic models of inflammation. The present study was undertaken with an objective to evaluate the anti-inflammatory activity of polyamines in different models of acute, subacute and chronic biochemical parameters. Several have also been investigated to correlate them with the anti-inflammatory activity of polyamines.

 » Materials and Methods Top


Wistar strain rats (120-150 g) were purchased from the registered breeder Bharath Sera Pvt Ltd., Mumbai, India. The rats were housed hygienically under standard conditions of temperature (24 ± 1 0 C), relative humidity (65 ± 10%) and light (10h) dark (14h) cycle. They were fed with standard pellet food (Amrut Laboratory animal feed diet, Maharashtra, India) and water ad lib .

Drugs, chemicals and reagents

Freund's adjuvant (complete) was a kind gift sample from Bharat Sera Vaccines, India. Carrageenin was purchased from Sigma-Aldrich, USA. Kits for SGOT and SGPT determination were purchased from Span diagnostics, Gujarat, India. All other chemicals used were of analytical reagent grade and were procured from local suppliers.

Animal models

Institutional animal ethics committee approval

All experimental protocols were approved by the institutional ethics committee.

Acute inflammation - Carrageenin induced hind paw edema in rats: Edema was produced acutely by injecting subcutaneously (s.c) 0.1 mL of carrageenin (1% w/v), into the plantar region of the hind paws of the rat according to the method of Winter et al . [7] Male wistar rats weighing between 120-150 g were used. A mark was made on both hind paws just below the tibiotarsal junction so that the paw could be dipped in the mercury column of the plethysmometer upto the mark to ensure constant paw volume. Putrescine (100 mg/kg), spermidine (17.5 mg/kg), spermine (12.5 mg/kg) and mixture of polyamines (100 mg/kg of putrescine + 17.5 mg/kg of spermidine + 12.5 mg/kg of spermine) were administered s.c in the neck region 3h prior to carrageenin injection [5] and paw volumes were measured at 2, 4 and 6 h after carrageenin injection. A control group treated with the vehicle (0.1 mL saline per 100 g) was used for comparison. Animals were sacrificed under light ether anesthesia at the end of 6 h, blood was collected by cardiac puncture for biochemical determinations like SGOT and SGPT by the method of Reitman and Frankel [8] and lipid peroxides in liver homogenates by the method of Ohkawa et al . [9] Paw edema was calculated for each rat by subtracting the baseline reading from that of 2, 4 and 6 hour. The anti-inflammatory activity of polyamines was determined by the following formula:

2.3.2 Sub-acute inflammation - Cotton pellet granuloma in rats: The cotton pellet granuloma was produced in rats by the method of Winter and Porter with slight modification. [10] The pellets, weighing exactly 10 mg each, were made from 5 mm sections of cotton rolls. The cotton pellets were sterilized in an autoclave for 30 minutes at 120 0 C under 15 lb pressure. Four pellets were inserted s.c into the ventral region, two on either side, in each rat under light ether anesthesia. Vehicle (0.1mL saline per 100 g), putrescine (25 mg/kg), spermidine (7 mg/kg), spermine (5 mg/kg) and mixture of polyamines (25 mg/kg of putrescine + 7 mg/kg of spermidine + 5 mg/kg of spermine) were administered daily for 7 days subcutaneously in the neck region. Animals were sacrificed on the 8 th day, blood was collected by cardiac puncture for SGOT and SGPT determination. The liver was separated and lipid peroxides in liver homogenate were determined. The cotton pellets (along with the granular tissue formed around) were removed surgically and freed from extraneous tissue. The pellets were weighed immediately for wet weight. Then, pellets were dried in an incubator at 60 0 C until a constant weight was obtained (all the exudate dried up). The exudate amount (weight of exudate in mg) was calculated by subtracting the constant dry weight of pellet from the immediate wet weight of pellet. The granulation tissue formation (dry weight of granuloma) was calculated after deducting the weight of cotton pellet (10 mg) from the constant dry weight of pellet and taken as a measure of granuloma tissue formation. The percent inhibitions of exudate and granuloma tissue formation were determined as follows:

Chronic inflammation

Freund's adjuvant induced arthritis: Freund's adjuvant (0.1mL, complete) was injected s.c into the plantar region of the right hind paw of the rat according to the method described by Stoerk et al [11] and Weichman. [12] Paw volume was measured on every alternative day for 21 days using volume displacement plethysmometer. Vehicle (0.1 mL saline per 100 g rat), putrescine (12.5 mg/kg), spermidine (3.5 mg/kg), spermine (2.5 mg/kg) and mixture of polyamines (12.5 mg/kg of putrescine + 3.5 mg/kg of spermidine + 2.5 mg/kg of spermine) were administered s.c in the neck region from the day of adjuvant injection daily for 14 days and animals were observed for arthritic symptoms like primary lesion (injected paw), secondary lesions (non-injected paw swelling), knee joint movements and pain threshold (assessed by vocal reflex - squeaks), grip strength (assessed by holding capacity on a wire mesh inclined plane when varying weights are attached to its tail) and paw volume were measured on every alternative day for 21 days. The animals were sacrificed on 22 nd day and blood was collected by cardiac puncture for the determinations of SGOT, SGPT and the lipid peroxides in liver homogenate were also measured.

The quantitative estimation of lipid peroxidation was done by determining the concentration of thiobarbituric acid reactive substance (TBARS) in the liver using the method of Ohakawa et al . [9] The amount of malondialdehyde (MDA) formed was quantified by reaction with TBA and used as an index of lipid peroxidation. The results were expressed as nanomoles of MDA/g of wet liver using molar extinction coefficient of the chromophore (1.56 X 10 -5 M/cm).

Statistical analysis

One-way ANOVA with Dunnett's post test was performed using GraphPad InStat version 3.00 for Windows 95 (Graphpad Software, San Diego California USA)

 » Results Top

Individual polyamines elicited a significant anti-inflammatory activity in carrageenin edema test. However, a mixture of the three polyamines did not elicit a synergistic activity [Table 1].

Rats treated with individual polyamines showed a significant inhibition of granuloma tissue as well as exudate formation. Among them, spermine showed the maximum inhibition. Treatment with a mixture of three polyamines showed maximum inhibition of granuloma tissue formation as well as that of exudates [Table 2]. Individual polyamine treatment daily for 14 days in arthritic rats inhibited the primary and secondary lesions, decreased the pain threshold perception and improved the joint movement and grip function during 21 days observation period [Table 3]. However, individual polyamines exhibited varied degree of edema inhibition in the injected and non-injected paws [Figure 1].

The treatment with polyamines individually as well as together (mixture) inhibited significantly the increase in SGOT and SGPT levels in different types of experimental inflammation. The MDA content of the liver homogenate was increased significantly in all types of experimental inflammation as compared to normal group. Treatment with polyamines individually as well as together (mixture) significantly reversed the elevated lipid peroxides. The degree of reversal was slightly more with a mixture of polyamines. All individual polyamines reversed the increase in lipid peroxide formation in all types of inflammation [Table 4].

 » Discussion Top

The previous studies have indicated elevated polyamines levels in edematous and granulomatous tissue during acute and sub chronic experimental models of inflammation. [13] The increased polyamines levels are known to trigger negative immune regulators by their action on lymphocytes, neutrophil locomotion and natural killer cell activity. [14],[15],[16] Furthermore, the involvement of spermine in macrophage cytokine synthesis inhibition has also been reported. [17] These experimental findings clearly suggest that endogenous polyamines act as regulators of the inflammatory process. Our efforts were directed to observe the effect of exogenous polyamines on different types of experimental inflammation.

Our experiments showed that exogenous polyamines treatment either individually or as a mixture elicited a significant anti-inflammatory activity in carrageenin (acute), cotton pellet granuloma (sub-acute) and freunds-adjuvant polyarthritis rat models. Furthermore, polyamine treatment prevented the increase in lysosomal marker enzymes SGOT, SGPT and increased liver peroxides during different type of inflammatory conditions significantly. The inhibition of lysosomal marker enzymes may be largely due to membrane stabilizing property of polyamines. [18] Polyamines are known to exhibit antioxidant activity [19],[20],[21] and the same has been confirmed in our experiments too. It is likely that both antioxidant activity as well as a membrane stabilizing effect of polyamines might be contributing to their anti-inflammatory activity observed in different types of inflammation. The other plausible explanation may be due to a negative immune regulation via their effect on lymphocytes and/or neutrophil locomotion or natural cell killer activity. Furthermore, the direct involvement of polyamines in stimulation of the synthesis of vasoregulin, a known anti inflammatory protein by its direct action on leukocytes had been reported. [22] This inherent effect of polyamines cannot be ignored for the observed anti-inflammatory activity of polyamines.

In conclusion, our experimental results suggest that exogenous polyamines administered by subcutaneous route exhibit anti-inflammatory activity in acute and chronic inflammation. A combined treatment with three polyamines exhibits no synergistic activity. The possible mechanism of anti-inflammatory activity of polyamines may be due to their inherent anti-oxidant activity and/or lysosomal membrane stabilization as supported by our experimental findings. Thus, studies on polyamines may be helpful in developing a new approach for better understanding of the inflammatory process and the generation of new anti-inflammatory drugs.

 » References Top

1.Lovas E, Carlin G. Spermine: An anti-oxidant and anti-inflammatory agent. Free Radical Biol Med 1991;11:455-61.  Back to cited text no. 1    
2.Tabor CW, Tabor H. 1, 4-Diaminobutane (putrescine), spermidine and spermine. Ann Rev Biochem 1976;45:285-306.  Back to cited text no. 2  [PUBMED]  [FULLTEXT]
3.Igarashi K, Kashiwagi K. Polyamines: Mysterious modulators of cellular functions. Biochem Biophys Res Commun 2000;271:559-64.  Back to cited text no. 3  [PUBMED]  [FULLTEXT]
4.Chattopadhyay MK, Tabor CW, Tabor H. Polyamines protect Escherichia coli cells from the toxic effect of oxygen. Proc Natl Acad Sci USA 2003;100:2261-5.  Back to cited text no. 4  [PUBMED]  [FULLTEXT]
5.Oyanagui Y. Anti-inflammatory effects of polyamines in serotonin and carrageenin paw edemata-possible mechanisms to increase vascular permeability inhibitory protein level which is regulated by glucocorticoids and superoxide radical. Agents Actions 1984;14:228-37.  Back to cited text no. 5  [PUBMED]  
6.Theoharides TC. Polyamines spermidine and spermine as modulators of calcium-dependent immune processes. Life Sci 1980;27:703-13.  Back to cited text no. 6  [PUBMED]  
7.Winter CA, Risley EA, Nuss GW. Carrageenin induced oedema in hind paws of the rat as an assay for anti-inflammatory drugs. Proc Soc Exp Biol Med 1962;111:544-7.  Back to cited text no. 7  [PUBMED]  
8.Reitman S, Frankel S. Colorimetric method for determination of glutamate oxaloacetate and glutamic pyruvic transaminase. Am J Clin Pathol 1957;28:56-62.  Back to cited text no. 8    
9.Ohkawa H, Ohishi N, Yagi K. Assay for lipid peroxides in animal tissues by thiobarbituric acid reaction. Anal Biochem 1979;95:351-8.  Back to cited text no. 9  [PUBMED]  
10.Winter CA, Porter CC. Effect of alterations in side chain upon anti-inflammatory and liver glycogen activities of hydrocortisone esters. J Am Pharm Assoc 1957;46:515-20.  Back to cited text no. 10    
11.Stoerk HC, Bielinski TC, Budzilovich T. Chronic polyarthritis in rats injected with spleen in adjuvants. Am J Pathol 1954;30:616-21.  Back to cited text no. 11    
12.Weichman BM. Rat adjuvant arthritis: A model of chronic inflammation in pharmacological methods in the control of inflammation. In: Chang JY, Lewis AJ, editors. New York: Alan Liss Inc; p. 363-80.  Back to cited text no. 12    
13.Lagishetty Chakradhar V, Naik SR. Polyamines in inflammation and their modulation by conventional anti-inflammatory drugs. Indian J Exp Biol 2007;45:649-53.  Back to cited text no. 13    
14.Ferrante A, Maxwell GM, Rencis VO. Inhibition of the respiratory burst of human neutrophils by the polyamine oxidase-polyamine system. Int J Immunopharmacol 1986;8:411-7.  Back to cited text no. 14    
15.Quemener V, Blanchard Y, Chamaillard L. Polyamine deprivation: A new tool in cancer treatment. Anticancer Res 1994;14:443-8.  Back to cited text no. 15    
16.Zhang M, Borovikova LV, Wang H. Spermine inhibition of monocyte activation and inflammation. Mol Med 1999;5:595-605.  Back to cited text no. 16    
17.Zhang M, Caragine T, Wang H, Cohen PS, Botchkina G, Soda K, et al. Spermine inhibits proinflammatory cytokine synthesis in human mononuclear cells: A counter regulatory mechanism that restrains the immune response. J Exp Med 1997;185:1759-68.  Back to cited text no. 17  [PUBMED]  [FULLTEXT]
18.Powroznik B, Gharbi M, Dandrifosse G, Peulen O. Enhancement of lysozyme stability and activity by polyamines. Biochimie 2004;86:651-6.  Back to cited text no. 18  [PUBMED]  [FULLTEXT]
19.Ohmori S, Misaizu T, Kitada M, Kitawaga H, Igarashi K, Hirose S, et al. Polyamine lowered the hepatic lipid peroxide level in rats. Res Commun Chem Pathol Pharmacol 1988;62:235-49.  Back to cited text no. 19    
20.Kafy AM, Lewis DA. Anti-oxidant effects of exogenous polyamines in damage of lysosomes inflicted by xanthine oxidase or stimulated polymorphonuclear leucocytes. Agents Actions 1988;24:145-51.  Back to cited text no. 20  [PUBMED]  
21.Tadolini B. Polyamine inhibition of lipoperoxidation. Biochem J 1988;249:33-6.  Back to cited text no. 21  [PUBMED]  [FULLTEXT]
22.Azuma Y, Ohtani S, Matsunaga Y, Ueda Y, Tajima K, Takagi N. Possible mechanisms of the anti-inflammatory action of polyamines. Gifu Shika Gakkai Zasshi 1990;17:509-15.  Back to cited text no. 22  [PUBMED]  


  [Figure 1]

  [Table 1], [Table 2], [Table 3], [Table 4]

This article has been cited by
1 Nutrigenomics of inward rectifier potassium channels
Gonzalo Ferreira, Axel Santander, Romina Cardozo, Luisina Chavarría, Lucía Domínguez, Nicolás Mujica, Milagros Benítez, Santiago Sastre, Luis Sobrevia, Garth L. Nicolson
Biochimica et Biophysica Acta (BBA) - Molecular Basis of Disease. 2023; : 166803
[Pubmed] | [DOI]
2 Polyamines in Parkinson's Disease: Balancing Between Neurotoxicity and Neuroprotection
Stephanie Vrijsen, Marine Houdou, Ana Cascalho, Jan Eggermont, Peter Vangheluwe
Annual Review of Biochemistry. 2023; 92(1): 435
[Pubmed] | [DOI]
3 In vitro ruminal fermentation and cow-to-mouse fecal transplantations verify the inter-relationship of microbiome and metabolome biomarkers: potential to promote health in dairy cows
Jui-Chun Hsieh, Shih-Te Chuang, Yu-Ting Hsu, Shang-Tse Ho, Kuan-Yi Li, Shih-Hsuan Chou, Ming-Ju Chen
Frontiers in Veterinary Science. 2023; 10
[Pubmed] | [DOI]
4 Overview of Polyamines as Nutrients for Human Healthy Long Life and Effect of Increased Polyamine Intake on DNA Methylation
Kuniyasu Soda
Cells. 2022; 11(1): 164
[Pubmed] | [DOI]
5 Expression of Polyamine Oxidase in Fibroblasts Induces MMP-1 and Decreases the Integrity of Extracellular Matrix
Hae Dong Jeong, Jin Hyung Kim, Go Eun Kwon, Seung-Taek Lee
International Journal of Molecular Sciences. 2022; 23(18): 10487
[Pubmed] | [DOI]
6 Metabolomic Profiling of Respiratory Muscles and Lung in Response to Long-Term Controlled Mechanical Ventilation
Ya Wen, Xiang Zhang, Lars Larsson
Frontiers in Cell and Developmental Biology. 2022; 10
[Pubmed] | [DOI]
7 Inter-organellar Communication in Parkinson's and Alzheimer's Disease: Looking Beyond Endoplasmic Reticulum-Mitochondria Contact Sites
Stephanie Vrijsen, Céline Vrancx, Mara Del Vecchio, Johannes V. Swinnen, Patrizia Agostinis, Joris Winderickx, Peter Vangheluwe, Wim Annaert
Frontiers in Neuroscience. 2022; 16
[Pubmed] | [DOI]
8 Helping the helpers: polyamines help maintain helper T-cell lineage fidelity
Tracy Murray Stewart, Cassandra E. Holbert, Robert A. Casero
Immunometabolism. 2022; 4(3): e00002
[Pubmed] | [DOI]
9 Glutathione, polyamine, and lysophosphatidylcholine synthesis pathways are associated with circulating pro-inflammatory cytokines
Ming Liu, Hongwei Zhang, Zikun Xie, Yiheng Huang, Guang Sun, Dake Qi, Andrew Furey, Edward W. Randell, Proton Rahman, Guangju Zhai
Metabolomics. 2022; 18(10)
[Pubmed] | [DOI]
10 Novel aspects of age-protection by spermidine supplementation are associated with preserved telomere length
Alexander Wirth, Bettina Wolf, Cheng-Kai Huang, Silke Glage, Sebastian J. Hofer, Marion Bankstahl, Christian Bär, Thomas Thum, Kai G. Kahl, Stephan J. Sigrist, Frank Madeo, Jens P. Bankstahl, Evgeni Ponimaskin
GeroScience. 2021; 43(2): 673
[Pubmed] | [DOI]
11 The metabolic role of spermidine in obesity: Evidence from cells to community
Yanee Choksomngam, Sintip Pattanakuhar, Nipon Chattipakorn, Siriporn C. Chattipakorn
Obesity Research & Clinical Practice. 2021; 15(4): 315
[Pubmed] | [DOI]
12 Probiotics Bacillus licheniformis Improves Intestinal Health of Subclinical Necrotic Enteritis-Challenged Broilers
Liugang Kan, Fangshen Guo, Yan Liu, Van Hieu Pham, Yuming Guo, Zhong Wang
Frontiers in Microbiology. 2021; 12
[Pubmed] | [DOI]

The Anti-Inflammatory Effect of Different Doses of Aliskiren in Rat Models of Inflammation

Tavga Ahmed Aziz, Ahmed Azad Kareem, Hemn Hassan Othman, Zheen Aorahman Ahmed
Drug Design, Development and Therapy. 2020; Volume 14: 2841
[Pubmed] | [DOI]
14 Polyamines, folic acid supplementation and cancerogenesis
Gordana Bjelakovic, Ivana Stojanovic, Tatjana Jevtovic Stoimenov, Dusica Pavlovic, Gordana Kocic, Goran B. Bjelakovic, Dusan Sokolovic, Jelena Basic
Pteridines. 2017; 28(3-4): 115
[Pubmed] | [DOI]
15 The Nutrient and Metabolite Profile of 3 Complementary Legume Foods with Potential to Improve Gut Health in Rural Malawian Children
Erica C Borresen, Lei Zhang, Indi Trehan, Nora Jean Nealon, Kenneth M Maleta, Mark J Manary, Elizabeth P Ryan
Current Developments in Nutrition. 2017; 1(10): 1010001
[Pubmed] | [DOI]
16 A co culture approach show that polyamine turnover is affected during inflammation in Atlantic salmon immune and liver cells and that arginine and LPS exerts opposite effects on p38MAPK signaling
Elisabeth Holen,Marit Espe,Synne M. Andersen,Richard Taylor,Anders Aksnes,Zebasil Mengesha,Pedro Araujo
Fish & Shellfish Immunology. 2014; 37(2): 286
[Pubmed] | [DOI]
17 Health effects and occurrence of dietary polyamines: A review for the period 2005 - mid 2013
Pavel Kalac
Food Chemistry. 2014;
[Pubmed] | [DOI]
18 In vitro antioxidant activity of dietary polyamines
Natalia Toro-Funes,Joan Bosch-Fusté,M. Teresa Veciana-Nogués,Maria Izquierdo-Pulido,M. Carmen Vidal-Carou
Food Research International. 2013; 51(1): 141
[Pubmed] | [DOI]
19 Synthesis, cytotoxicity, and in vitro antileishmanial activity of mono-t-butyloxycarbonyl-protected diamines
Alessandra C. Pinheiro, Marcele N. Rocha, Paula M. Nogueira, Thaís C.M. Nogueira, Liana F. Jasmim, Marcus V.N. de Souza, Rodrigo P. Soares
Diagnostic Microbiology and Infectious Disease. 2011;
[VIEW] | [DOI]
20 Polyamines in foods: development of a food database
Eric Poortvliet, Mohamed Atiya Ali, Roger Strömberg, Agneta Yngve
Food & Nutrition Research. 2011; 55(0)
[VIEW] | [DOI]
21 Nitrosative stress and apoptosis in experimental alcoholic hepatitis. An immunohistochemical study
Stǎnciulescu, E.C., Mitrea, N., Pisoschi, C., Banitǎ, M., Purcaru, O., Tache, D., Rǎu, G., Arsene, A.
Farmacia. 2010; 58(1): 70-77
22 Pyrazoles as promising scaffold for the synthesis of anti-inflammatory and/or antimicrobial agent: A review
Bekhit, A.A., Hymete, A., Bekhit, A.E.A., Damtew, A., Aboul-Enein, H.Y.
Mini-Reviews in Medicinal Chemistry. 2010; 10(11): 1014-1033
23 Recent advances in the research on biological roles of dietary polyamines in man
Pavel Kalac
Journal of Applied Biomedicine. 2009; 7(2): 65
[Pubmed] | [DOI]
24 Recent advances in the research on biological roles of dietary polyamines in man
Kalač, P.
Journal of Applied Biomedicine. 2009; 7(2): 65-74


Print this article  Email this article


Site Map | Home | Contact Us | Feedback | Copyright and Disclaimer | Privacy Notice
Online since 20th July '04
Published by Wolters Kluwer - Medknow