| RESEARCH PAPER
|Year : 2007 | Volume
| Issue : 2 | Page : 75-79
Role of 5-HT 1A receptors in antidepressant-like effect of dichloromethane fraction of Kielmeyera coriacea in rats subjected to the forced swim test
FJ Otobone, VR Sela, S Obici, LY Moreira, DAG Cortez, EA Audi
Department of Pharmacy and Pharmacology, University of Maringá, PR, Brazil
Objective : We examined the involvement of 5-HT neurotransmission on the antidepressant-like effect of the dichloromethane (DcM) fraction of an extract from Kielmeyera coriacea stems.
Materials and Methods : Male Wistar rats treated chronically (45 days, gavage) with the DcM fraction received an intradorsal raphe nucleus (DRN) microinjection of saline or 5-HT 1A receptor ligands and were evaluated in the forced swimming test (FST) and in the open-field test (OFT).
Results : The DcM fraction (5.0 mg/kg) reduced immobility time in the FST without altering locomotion in the OFT. IntraDRN microinjection of the 5-HT 1A receptor agonist, (+)-8-OH-DPAT (0.10; 0.20 or 0.33 µg) increased immobility time and reduced locomotion at the higher dose whereas the 5-HT1A antagonists, (-)-pindolol (0.10; 0.20 or 0.40 µg) or WAY100635 (0.11; 0.22 or 0.43 µg) did not produce any effect in the behavioral tests. IntraDRN (+)-8-OH-DPAT (0.20 or 0.33 µg) in rats treated with the DcM fraction (5.0 mg/kg) blocked the changes in the immobility time or in locomotion produced by each drug. Intra-DRN (-)-pindolol (0.10 µg) or WAY100635 (0.43 µg) in rats treated with a subactive dose of the DcM fraction (4.0 mg/kg) synergistically reduced immobility time in the FST.
Conclusion : The DcM fraction of Kielmeyera coriacea produced an antidepressant-like effect in the FST and interacted with 5-HT 1A receptor ligands. Activation of 5-HT 1A receptors into DRN by (+) 8-OH-DPAT produced detectable changes in the FST or in the OFT.
E A Audi
Department of Pharmacy and Pharmacology, University of Maringá, PR
Source of Support: None, Conflict of Interest: None
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